TY - JOUR
T1 - Optimization of fermentation parameters to enhance the production of ethanol from palmyra jaggery using Saccharomyces cerevisiae in a batch fermentor
AU - Bandaru, Veera Venkata Ratnam
AU - Bandaru, Sita Ramalakshmi
AU - Somalanka, Subba Rao
AU - Mendu, Damodara Rao
AU - Imandi, Sarat Babu
AU - Bejawada, Srinivasa Rao
AU - Medicherla, Narasimha Rao
AU - Devarajan, Thangadurai
AU - Karothi, Jayaraju
AU - Chityala, Ayyanna
PY - 2007/12
Y1 - 2007/12
N2 - Application of statistical experimental designs for optimization of fermentation parameters to enhance ethanol production, which is an economical and renewable energy source using Saccharomyces cerevisiae NCIM 3090 from palmyra jaggery, was studied in a batch fermentor. Using Plackett-Burman design, impeller speed, concentrations of CoCl2 and KH2PO4 were identified as significant variables, which highly influenced ethanol production, and these variables were further optimized using a central composite design (CCD). The ethanol production was adequately approximated with a full quadratic equation obtained from three factors and five levels of CCD. Maximum ethanol concentration of 132.56 g/l (16.8% [v/v]) was obtained for an impeller speed of 247.179 (≈250) rev/min, CoCl2 of 0.263 g/l and KH2PO4 of 2.39 g/l. A second-order polynomial regression model was fitted and was found adequate with R 2 of 0.8952. This combined statistical approach enables rapid identification and investigation of significant parameters for improving the ethanol production and could be very useful in optimizing processes.
AB - Application of statistical experimental designs for optimization of fermentation parameters to enhance ethanol production, which is an economical and renewable energy source using Saccharomyces cerevisiae NCIM 3090 from palmyra jaggery, was studied in a batch fermentor. Using Plackett-Burman design, impeller speed, concentrations of CoCl2 and KH2PO4 were identified as significant variables, which highly influenced ethanol production, and these variables were further optimized using a central composite design (CCD). The ethanol production was adequately approximated with a full quadratic equation obtained from three factors and five levels of CCD. Maximum ethanol concentration of 132.56 g/l (16.8% [v/v]) was obtained for an impeller speed of 247.179 (≈250) rev/min, CoCl2 of 0.263 g/l and KH2PO4 of 2.39 g/l. A second-order polynomial regression model was fitted and was found adequate with R 2 of 0.8952. This combined statistical approach enables rapid identification and investigation of significant parameters for improving the ethanol production and could be very useful in optimizing processes.
KW - Batch fermentor
KW - Central composite design
KW - Ethanol
KW - Palmyra jaggery
KW - Plackett-Burman design
KW - Saccharomyces cerevisiae
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U2 - 10.1007/s12010-007-0048-x
DO - 10.1007/s12010-007-0048-x
M3 - Article
C2 - 18057450
AN - SCOPUS:36949039617
SN - 0273-2289
VL - 143
SP - 224
EP - 235
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 3
ER -