Optimization of fermentation parameters to enhance the production of ethanol from palmyra jaggery using Saccharomyces cerevisiae in a batch fermentor

Veera Venkata Ratnam Bandaru, Sita Ramalakshmi Bandaru, Subba Rao Somalanka, Damodara Rao Mendu, Sarat Babu Imandi, Srinivasa Rao Bejawada, Narasimha Rao Medicherla, Thangadurai Devarajan, Jayaraju Karothi, Ayyanna Chityala

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6 Scopus citations


Application of statistical experimental designs for optimization of fermentation parameters to enhance ethanol production, which is an economical and renewable energy source using Saccharomyces cerevisiae NCIM 3090 from palmyra jaggery, was studied in a batch fermentor. Using Plackett-Burman design, impeller speed, concentrations of CoCl2 and KH2PO4 were identified as significant variables, which highly influenced ethanol production, and these variables were further optimized using a central composite design (CCD). The ethanol production was adequately approximated with a full quadratic equation obtained from three factors and five levels of CCD. Maximum ethanol concentration of 132.56 g/l (16.8% [v/v]) was obtained for an impeller speed of 247.179 (≈250) rev/min, CoCl2 of 0.263 g/l and KH2PO4 of 2.39 g/l. A second-order polynomial regression model was fitted and was found adequate with R 2 of 0.8952. This combined statistical approach enables rapid identification and investigation of significant parameters for improving the ethanol production and could be very useful in optimizing processes.

Original languageEnglish (US)
Pages (from-to)224-235
Number of pages12
JournalApplied Biochemistry and Biotechnology
Issue number3
Publication statusPublished - Dec 1 2007



  • Batch fermentor
  • Central composite design
  • Ethanol
  • Palmyra jaggery
  • Plackett-Burman design
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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