Analysis of regiospecific triacylglycerols by electrospray ionization-mass spectrometry3 of lithiated adducts

Jiann Tsyh Lin, Arthur J. Arcinas

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A method of regiospecific analysis of triacylglycerols (TAGs) in vegetable oils and animal fats is reported here using the electrospray ionization-mass spectrometry (MS3) of TAG-lithiated adducts. The fragment ions of the MS3 from the loss of fatty acids at the sn-2 position as α,β-unsaturated fatty acids were used for regiospecific identification and quantification. The ratio of the regiospecific TAGs, ABA and AAB, in an oil sample usually fraction collected by high-performance liquid chromatography can be determined by the abundance of the fragment ions of [ABA + Li - ACOOH - B′CH=CHCOOH]+ and [AAB + Li - ACOOH - A′CH=CHCOOH]+. The method was used to analyze regiospecific TAGs in extra virgin olive oil. The results showed that the saturated fatty acids, palmitic and stearic acids, were mostly located at the sn-1,3 positions and unsaturated fatty acids, oleic and linoleic acids, were mostly located at the sn-2 position.

Original languageEnglish (US)
Pages (from-to)4909-4915
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume56
Issue number13
DOIs
StatePublished - Jul 9 2008
Externally publishedYes

Fingerprint

Electrospray ionization
ionization
Triglycerides
triacylglycerols
Unsaturated Fatty Acids
unsaturated fatty acids
Fatty Acids
Stearic Acids
Ions
Oleic Acids
ions
Linoleic Acids
extra-virgin olive oil
Plant Oils
animal fats and oils
Electrospray Ionization Mass Spectrometry
High performance liquid chromatography
stearic acid
vegetable oil
palmitic acid

Keywords

  • α,β-unsaturated fatty acids
  • Electrospray ionization
  • Lithiated adducts
  • Mass spectrometry
  • Olive oil
  • Regiospecific
  • Triacylglycerols

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Analysis of regiospecific triacylglycerols by electrospray ionization-mass spectrometry3 of lithiated adducts. / Lin, Jiann Tsyh; Arcinas, Arthur J.

In: Journal of Agricultural and Food Chemistry, Vol. 56, No. 13, 09.07.2008, p. 4909-4915.

Research output: Contribution to journalArticle

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