Analysis of regiospecific triacylglycerols by electrospray ionization-mass spectrometry3 of lithiated adducts

Jiann Tsyh Lin, Arthur Arcinas

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


A method of regiospecific analysis of triacylglycerols (TAGs) in vegetable oils and animal fats is reported here using the electrospray ionization-mass spectrometry (MS3) of TAG-lithiated adducts. The fragment ions of the MS3 from the loss of fatty acids at the sn-2 position as α,β-unsaturated fatty acids were used for regiospecific identification and quantification. The ratio of the regiospecific TAGs, ABA and AAB, in an oil sample usually fraction collected by high-performance liquid chromatography can be determined by the abundance of the fragment ions of [ABA + Li - ACOOH - B′CH=CHCOOH]+ and [AAB + Li - ACOOH - A′CH=CHCOOH]+. The method was used to analyze regiospecific TAGs in extra virgin olive oil. The results showed that the saturated fatty acids, palmitic and stearic acids, were mostly located at the sn-1,3 positions and unsaturated fatty acids, oleic and linoleic acids, were mostly located at the sn-2 position.

Original languageEnglish (US)
Pages (from-to)4909-4915
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Issue number13
StatePublished - Jul 9 2008
Externally publishedYes


  • Electrospray ionization
  • Lithiated adducts
  • Mass spectrometry
  • Olive oil
  • Regiospecific
  • Triacylglycerols
  • α,β-unsaturated fatty acids

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)


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