Zinc(II) inhibits the release of thyroid and glucocorticoid receptors from chromatin of cultured GC cells

I. J. Ramirez, M. Halwer, L. E. Shapiro, Martin I. Surks

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The effect of Zn(II) on the association of thyroid and glucocorticoid hormone receptors with chromatin was studied in chromatin from cultured GC cells. Chromatin was incubated at 0-4°C in 20 mM Tris, pH 7.4. When buffers contained 0.15 M NaCl, the release of T3 receptors from chromatin was time-dependent; 50% of T3 receptors were released after 30 min incubation. Receptor release appeared relatively specific since <10% of chromatin protein and DNA, and <13% of chromatin zinc were released under these conditions. Addition of Zn(II) inhibited receptor release; one-half maximal inhibition occurred at 1 μM ZnCl2. Cd(II) and to a lesser extent (Co(II) had similar but smaller effects. Addition of EDTA prevented this effect of Zn(II); EDTA alone enhanced receptor release. Zn(II) also inhibited the release of glucocorticoid receptors from chromatin in similar incubations. Our findings suggest that Zn(II) increases the association of hormone receptors with chromatin and, thereby, may influence receptor function.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalHormone and Metabolic Research
Volume23
Issue number4
StatePublished - 1991

Fingerprint

Glucocorticoid Receptors
Chromatin
Zinc
Cultured Cells
Thyroid Gland
Thyroid Hormone Receptors
Edetic Acid
Hormones
Glucocorticoids
Buffers
DNA

Keywords

  • Chromatin
  • GC cells
  • Glucocorticoid receptors
  • T receptors
  • Zinc

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Zinc(II) inhibits the release of thyroid and glucocorticoid receptors from chromatin of cultured GC cells. / Ramirez, I. J.; Halwer, M.; Shapiro, L. E.; Surks, Martin I.

In: Hormone and Metabolic Research, Vol. 23, No. 4, 1991, p. 155-161.

Research output: Contribution to journalArticle

@article{e509dd5322ae4fc68f2b87b0d0ba3bf7,
title = "Zinc(II) inhibits the release of thyroid and glucocorticoid receptors from chromatin of cultured GC cells",
abstract = "The effect of Zn(II) on the association of thyroid and glucocorticoid hormone receptors with chromatin was studied in chromatin from cultured GC cells. Chromatin was incubated at 0-4°C in 20 mM Tris, pH 7.4. When buffers contained 0.15 M NaCl, the release of T3 receptors from chromatin was time-dependent; 50{\%} of T3 receptors were released after 30 min incubation. Receptor release appeared relatively specific since <10{\%} of chromatin protein and DNA, and <13{\%} of chromatin zinc were released under these conditions. Addition of Zn(II) inhibited receptor release; one-half maximal inhibition occurred at 1 μM ZnCl2. Cd(II) and to a lesser extent (Co(II) had similar but smaller effects. Addition of EDTA prevented this effect of Zn(II); EDTA alone enhanced receptor release. Zn(II) also inhibited the release of glucocorticoid receptors from chromatin in similar incubations. Our findings suggest that Zn(II) increases the association of hormone receptors with chromatin and, thereby, may influence receptor function.",
keywords = "Chromatin, GC cells, Glucocorticoid receptors, T receptors, Zinc",
author = "Ramirez, {I. J.} and M. Halwer and Shapiro, {L. E.} and Surks, {Martin I.}",
year = "1991",
language = "English (US)",
volume = "23",
pages = "155--161",
journal = "Hormone and Metabolic Research",
issn = "0018-5043",
publisher = "Georg Thieme Verlag",
number = "4",

}

TY - JOUR

T1 - Zinc(II) inhibits the release of thyroid and glucocorticoid receptors from chromatin of cultured GC cells

AU - Ramirez, I. J.

AU - Halwer, M.

AU - Shapiro, L. E.

AU - Surks, Martin I.

PY - 1991

Y1 - 1991

N2 - The effect of Zn(II) on the association of thyroid and glucocorticoid hormone receptors with chromatin was studied in chromatin from cultured GC cells. Chromatin was incubated at 0-4°C in 20 mM Tris, pH 7.4. When buffers contained 0.15 M NaCl, the release of T3 receptors from chromatin was time-dependent; 50% of T3 receptors were released after 30 min incubation. Receptor release appeared relatively specific since <10% of chromatin protein and DNA, and <13% of chromatin zinc were released under these conditions. Addition of Zn(II) inhibited receptor release; one-half maximal inhibition occurred at 1 μM ZnCl2. Cd(II) and to a lesser extent (Co(II) had similar but smaller effects. Addition of EDTA prevented this effect of Zn(II); EDTA alone enhanced receptor release. Zn(II) also inhibited the release of glucocorticoid receptors from chromatin in similar incubations. Our findings suggest that Zn(II) increases the association of hormone receptors with chromatin and, thereby, may influence receptor function.

AB - The effect of Zn(II) on the association of thyroid and glucocorticoid hormone receptors with chromatin was studied in chromatin from cultured GC cells. Chromatin was incubated at 0-4°C in 20 mM Tris, pH 7.4. When buffers contained 0.15 M NaCl, the release of T3 receptors from chromatin was time-dependent; 50% of T3 receptors were released after 30 min incubation. Receptor release appeared relatively specific since <10% of chromatin protein and DNA, and <13% of chromatin zinc were released under these conditions. Addition of Zn(II) inhibited receptor release; one-half maximal inhibition occurred at 1 μM ZnCl2. Cd(II) and to a lesser extent (Co(II) had similar but smaller effects. Addition of EDTA prevented this effect of Zn(II); EDTA alone enhanced receptor release. Zn(II) also inhibited the release of glucocorticoid receptors from chromatin in similar incubations. Our findings suggest that Zn(II) increases the association of hormone receptors with chromatin and, thereby, may influence receptor function.

KW - Chromatin

KW - GC cells

KW - Glucocorticoid receptors

KW - T receptors

KW - Zinc

UR - http://www.scopus.com/inward/record.url?scp=0025854307&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025854307&partnerID=8YFLogxK

M3 - Article

VL - 23

SP - 155

EP - 161

JO - Hormone and Metabolic Research

JF - Hormone and Metabolic Research

SN - 0018-5043

IS - 4

ER -