Zinc(II) inhibits the release of thyroid and glucocorticoid receptors from chromatin of cultured GC cells

I. J. Ramirez, M. Halwer, L. E. Shapiro, M. I. Surks

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The effect of Zn(II) on the association of thyroid and glucocorticoid hormone receptors with chromatin was studied in chromatin from cultured GC cells. Chromatin was incubated at 0-4°C in 20 mM Tris, pH 7.4. When buffers contained 0.15 M NaCl, the release of T3 receptors from chromatin was time-dependent; 50% of T3 receptors were released after 30 min incubation. Receptor release appeared relatively specific since <10% of chromatin protein and DNA, and <13% of chromatin zinc were released under these conditions. Addition of Zn(II) inhibited receptor release; one-half maximal inhibition occurred at 1 μM ZnCl2. Cd(II) and to a lesser extent (Co(II) had similar but smaller effects. Addition of EDTA prevented this effect of Zn(II); EDTA alone enhanced receptor release. Zn(II) also inhibited the release of glucocorticoid receptors from chromatin in similar incubations. Our findings suggest that Zn(II) increases the association of hormone receptors with chromatin and, thereby, may influence receptor function.

Original languageEnglish (US)
Pages (from-to)155-161
Number of pages7
JournalHormone and Metabolic Research
Volume23
Issue number4
DOIs
StatePublished - Jan 1 1991

Keywords

  • Chromatin
  • GC cells
  • Glucocorticoid receptors
  • T receptors
  • Zinc

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Fingerprint Dive into the research topics of 'Zinc(II) inhibits the release of thyroid and glucocorticoid receptors from chromatin of cultured GC cells'. Together they form a unique fingerprint.

  • Cite this