Voriconazole inhibits melanization in Cryptococcus neoformans

Luis R. Martinez, Patricia Ntiamoah, Attila Gácser, Arturo Casadevall, Joshua D. Nosanchuk

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Voriconazole is a triazole antifungal drug that inhibits ergosterol synthesis and has broad activity against yeast and molds. While studying the interaction of voriconazole and Cryptococcus neoformans, we noted that cells grown in the presence of subinhibitory concentrations of voriconazole reduced melanin pigmentation. We investigated this effect systematically by assessing melanin production in the presence of voriconazole, amphotericin B, caspofungin, itraconazole, and fluconazole. Only voriconazole impeded the formation of melanin at subinhibitory concentrations. Voriconazole did not affect the autopolymerization of L-dopa, and 0.5 MIC of voriconazole did affect the gene expression of C. neoformans. However, voriconazole inhibited the capacity of laccase to catalyze the formation of melanin. Hence, voriconazole affects melanization in C. neoformans by interacting directly with laccase, which may increase the efficacy of this potent antifungal against certain pigmented fungi.

Original languageEnglish (US)
Pages (from-to)4396-4400
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume51
Issue number12
DOIs
StatePublished - Dec 2007

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Cryptococcus neoformans
Melanins
Laccase
caspofungin
Fungi
Voriconazole
Ergosterol
Triazoles
Itraconazole
Fluconazole
Pigmentation
Levodopa
Amphotericin B
Yeasts
Gene Expression

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Voriconazole inhibits melanization in Cryptococcus neoformans. / Martinez, Luis R.; Ntiamoah, Patricia; Gácser, Attila; Casadevall, Arturo; Nosanchuk, Joshua D.

In: Antimicrobial Agents and Chemotherapy, Vol. 51, No. 12, 12.2007, p. 4396-4400.

Research output: Contribution to journalArticle

Martinez, Luis R. ; Ntiamoah, Patricia ; Gácser, Attila ; Casadevall, Arturo ; Nosanchuk, Joshua D. / Voriconazole inhibits melanization in Cryptococcus neoformans. In: Antimicrobial Agents and Chemotherapy. 2007 ; Vol. 51, No. 12. pp. 4396-4400.
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