Visualization of fibrillar amyloid deposits in living, transgenic Caenorhabditis elegans animals using the sensitive amyloid dye, X-34

Christopher D. Link, Carolyn J. Johnson, Virginia Fonte, Marie Christine Paupard, David H. Hall, Scot Styren, Chester A. Mathis, William E. Klunk

Research output: Contribution to journalArticle

113 Scopus citations


Transgenic Caenorhabditis elegans animals can be engineered to express high levels of the human beta amyloid peptide (Abeta). Histochemistry of fixed tissue from these animals reveals deposits reactive with the amyloid-specific dyes Congo Red and thioflavin S (Fay et al., J. Neurochem 71:1616, 1998). Here we show by immuno-electron microscopy that these animals contain intracellular immunoreactive deposits with classic amyloid fibrillar ultrastructure. These deposits can be visualized in living animals using the newly developed, intensively fluorescent, amyloid-specific dye X-34. This in vivo staining allows monitoring of amyloid deposition in individual animals over time. The specificity of this staining is demonstrated by examining transgenic animals expressing high levels of a non-fibrillar beta peptide variant, the beta single-chain dimer. These animals have deposits immunoreactive with anti-beta antibodies, but do not have X-34 deposits or deposits with a fibrillar ultrastructure. X-34 can also be used in vivo to visualize putative amyloid deposits resulting from accumulation of human transthyretin, another amyloidic protein. In vivo amyloid staining with X-34 may be a useful tool for monitoring anti-amyloidic treatments in real time or screening for genetic alterations that affect amyloid formation.

Original languageEnglish (US)
Pages (from-to)217-226
Number of pages10
JournalNeurobiology of Aging
Issue number2
StatePublished - Feb 28 2001



  • 4G8
  • Abeta
  • Amyloid
  • BSB
  • C. elegans
  • Fibrillar ultrastructure
  • Immuno-EM
  • In vivo staining
  • Transgenic
  • Transthyretin
  • X-34

ASJC Scopus subject areas

  • Neuroscience(all)
  • Aging
  • Clinical Neurology
  • Developmental Biology
  • Geriatrics and Gerontology

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