Vinblastine paracrystals from cultured cells are calcium-stable

James J. Manfredi, Susan Band Horwitz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Vinblastine has two distinct tubulin-related effects in 3T6 fibroblasts and J774.2 macrophages. It depolymerizes microtubules and it induces the formation of paracrystals in the cytoplasm. These paracrystals are retained in cytoskeletons prepared by Triton extraction and are stable to treatment with calcium. The direct addition of vinblastine to cytoskeletons does not alter the organization of microtubules. The two effects of vinblastine are concentration-dependent, as assayed by binding of [3H]taxol and tubulin immunofluorescence. At low concentrations, vinblastine depolymerizes cellular microtubules; at high concentrations the drug induces the formation of paracrystals.

Original languageEnglish (US)
Pages (from-to)205-212
Number of pages8
JournalExperimental Cell Research
Volume150
Issue number1
DOIs
StatePublished - 1984

Fingerprint

Vinblastine
Cultured Cells
Microtubules
Calcium
Tubulin
Cytoskeleton
Paclitaxel
Fluorescent Antibody Technique
Cytoplasm
Fibroblasts
Macrophages
Pharmaceutical Preparations
Therapeutics

ASJC Scopus subject areas

  • Cell Biology

Cite this

Vinblastine paracrystals from cultured cells are calcium-stable. / Manfredi, James J.; Band Horwitz, Susan.

In: Experimental Cell Research, Vol. 150, No. 1, 1984, p. 205-212.

Research output: Contribution to journalArticle

@article{5a870fd020d644fcafdb80ce14b5fb89,
title = "Vinblastine paracrystals from cultured cells are calcium-stable",
abstract = "Vinblastine has two distinct tubulin-related effects in 3T6 fibroblasts and J774.2 macrophages. It depolymerizes microtubules and it induces the formation of paracrystals in the cytoplasm. These paracrystals are retained in cytoskeletons prepared by Triton extraction and are stable to treatment with calcium. The direct addition of vinblastine to cytoskeletons does not alter the organization of microtubules. The two effects of vinblastine are concentration-dependent, as assayed by binding of [3H]taxol and tubulin immunofluorescence. At low concentrations, vinblastine depolymerizes cellular microtubules; at high concentrations the drug induces the formation of paracrystals.",
author = "Manfredi, {James J.} and {Band Horwitz}, Susan",
year = "1984",
doi = "10.1016/0014-4827(84)90715-8",
language = "English (US)",
volume = "150",
pages = "205--212",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Vinblastine paracrystals from cultured cells are calcium-stable

AU - Manfredi, James J.

AU - Band Horwitz, Susan

PY - 1984

Y1 - 1984

N2 - Vinblastine has two distinct tubulin-related effects in 3T6 fibroblasts and J774.2 macrophages. It depolymerizes microtubules and it induces the formation of paracrystals in the cytoplasm. These paracrystals are retained in cytoskeletons prepared by Triton extraction and are stable to treatment with calcium. The direct addition of vinblastine to cytoskeletons does not alter the organization of microtubules. The two effects of vinblastine are concentration-dependent, as assayed by binding of [3H]taxol and tubulin immunofluorescence. At low concentrations, vinblastine depolymerizes cellular microtubules; at high concentrations the drug induces the formation of paracrystals.

AB - Vinblastine has two distinct tubulin-related effects in 3T6 fibroblasts and J774.2 macrophages. It depolymerizes microtubules and it induces the formation of paracrystals in the cytoplasm. These paracrystals are retained in cytoskeletons prepared by Triton extraction and are stable to treatment with calcium. The direct addition of vinblastine to cytoskeletons does not alter the organization of microtubules. The two effects of vinblastine are concentration-dependent, as assayed by binding of [3H]taxol and tubulin immunofluorescence. At low concentrations, vinblastine depolymerizes cellular microtubules; at high concentrations the drug induces the formation of paracrystals.

UR - http://www.scopus.com/inward/record.url?scp=0021364749&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021364749&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(84)90715-8

DO - 10.1016/0014-4827(84)90715-8

M3 - Article

C2 - 6141062

AN - SCOPUS:0021364749

VL - 150

SP - 205

EP - 212

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -