Abstract
Vinblastine has two distinct tubulin-related effects in 3T6 fibroblasts and J774.2 macrophages. It depolymerizes microtubules and it induces the formation of paracrystals in the cytoplasm. These paracrystals are retained in cytoskeletons prepared by Triton extraction and are stable to treatment with calcium. The direct addition of vinblastine to cytoskeletons does not alter the organization of microtubules. The two effects of vinblastine are concentration-dependent, as assayed by binding of [3H]taxol and tubulin immunofluorescence. At low concentrations, vinblastine depolymerizes cellular microtubules; at high concentrations the drug induces the formation of paracrystals.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 205-212 |
| Number of pages | 8 |
| Journal | Experimental Cell Research |
| Volume | 150 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1984 |
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- Cell Biology
Cite this
Vinblastine paracrystals from cultured cells are calcium-stable. / Manfredi, James J.; Band Horwitz, Susan.
In: Experimental Cell Research, Vol. 150, No. 1, 1984, p. 205-212.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Vinblastine paracrystals from cultured cells are calcium-stable
AU - Manfredi, James J.
AU - Band Horwitz, Susan
PY - 1984
Y1 - 1984
N2 - Vinblastine has two distinct tubulin-related effects in 3T6 fibroblasts and J774.2 macrophages. It depolymerizes microtubules and it induces the formation of paracrystals in the cytoplasm. These paracrystals are retained in cytoskeletons prepared by Triton extraction and are stable to treatment with calcium. The direct addition of vinblastine to cytoskeletons does not alter the organization of microtubules. The two effects of vinblastine are concentration-dependent, as assayed by binding of [3H]taxol and tubulin immunofluorescence. At low concentrations, vinblastine depolymerizes cellular microtubules; at high concentrations the drug induces the formation of paracrystals.
AB - Vinblastine has two distinct tubulin-related effects in 3T6 fibroblasts and J774.2 macrophages. It depolymerizes microtubules and it induces the formation of paracrystals in the cytoplasm. These paracrystals are retained in cytoskeletons prepared by Triton extraction and are stable to treatment with calcium. The direct addition of vinblastine to cytoskeletons does not alter the organization of microtubules. The two effects of vinblastine are concentration-dependent, as assayed by binding of [3H]taxol and tubulin immunofluorescence. At low concentrations, vinblastine depolymerizes cellular microtubules; at high concentrations the drug induces the formation of paracrystals.
UR - http://www.scopus.com/inward/record.url?scp=0021364749&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021364749&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(84)90715-8
DO - 10.1016/0014-4827(84)90715-8
M3 - Article
C2 - 6141062
AN - SCOPUS:0021364749
VL - 150
SP - 205
EP - 212
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 1
ER -