Vinblastine paracrystals from cultured cells are calcium-stable

James J. Manfredi; Susan Band Horwitz

doi:10.1016/0014-4827(84)90715-8

Vinblastine paracrystals from cultured cells are calcium-stable

James J. Manfredi, Susan Band Horwitz

Molecular Pharmacology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Vinblastine has two distinct tubulin-related effects in 3T6 fibroblasts and J774.2 macrophages. It depolymerizes microtubules and it induces the formation of paracrystals in the cytoplasm. These paracrystals are retained in cytoskeletons prepared by Triton extraction and are stable to treatment with calcium. The direct addition of vinblastine to cytoskeletons does not alter the organization of microtubules. The two effects of vinblastine are concentration-dependent, as assayed by binding of [³H]taxol and tubulin immunofluorescence. At low concentrations, vinblastine depolymerizes cellular microtubules; at high concentrations the drug induces the formation of paracrystals.

Original language	English (US)
Pages (from-to)	205-212
Number of pages	8
Journal	Experimental Cell Research
Volume	150
Issue number	1
DOIs	https://doi.org/10.1016/0014-4827(84)90715-8
State	Published - Jan 1984

ASJC Scopus subject areas

Cell Biology

Access to Document

10.1016/0014-4827(84)90715-8

Cite this

@article{5a870fd020d644fcafdb80ce14b5fb89,

title = "Vinblastine paracrystals from cultured cells are calcium-stable",

abstract = "Vinblastine has two distinct tubulin-related effects in 3T6 fibroblasts and J774.2 macrophages. It depolymerizes microtubules and it induces the formation of paracrystals in the cytoplasm. These paracrystals are retained in cytoskeletons prepared by Triton extraction and are stable to treatment with calcium. The direct addition of vinblastine to cytoskeletons does not alter the organization of microtubules. The two effects of vinblastine are concentration-dependent, as assayed by binding of [3H]taxol and tubulin immunofluorescence. At low concentrations, vinblastine depolymerizes cellular microtubules; at high concentrations the drug induces the formation of paracrystals.",

author = "Manfredi, {James J.} and Horwitz, {Susan Band}",

note = "Funding Information: We would like to thank Jane Fant, Pearl Grant, Jill Horowitz, Marian Meyers, George Orr, Jerome Pamess, Sharon Pifko-Hirst, Mary Rutigliano, Lynn Sorbara and Wilma Wasco for their comments and assistance.T his investigationw as supportedi n part by NIH grantsG M 29042a nd CA 15714a, nd ACS grant CH-86A. J. J. M. is the recipient of a fellowship from the Pharmaceuticalm anufacturers{\textquoteright} Association, and the data in this paper are from a thesis that was submittedi n partial fulfillmento f the degree of Doctor of Philosophy from the Sue Golding Graduate Division of the Albert Einstein College of Medicine, Bronx, N.Y.",

year = "1984",

month = jan,

doi = "10.1016/0014-4827(84)90715-8",

language = "English (US)",

volume = "150",

pages = "205--212",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Vinblastine paracrystals from cultured cells are calcium-stable

AU - Manfredi, James J.

AU - Horwitz, Susan Band

N1 - Funding Information: We would like to thank Jane Fant, Pearl Grant, Jill Horowitz, Marian Meyers, George Orr, Jerome Pamess, Sharon Pifko-Hirst, Mary Rutigliano, Lynn Sorbara and Wilma Wasco for their comments and assistance.T his investigationw as supportedi n part by NIH grantsG M 29042a nd CA 15714a, nd ACS grant CH-86A. J. J. M. is the recipient of a fellowship from the Pharmaceuticalm anufacturers’ Association, and the data in this paper are from a thesis that was submittedi n partial fulfillmento f the degree of Doctor of Philosophy from the Sue Golding Graduate Division of the Albert Einstein College of Medicine, Bronx, N.Y.

PY - 1984/1

Y1 - 1984/1

N2 - Vinblastine has two distinct tubulin-related effects in 3T6 fibroblasts and J774.2 macrophages. It depolymerizes microtubules and it induces the formation of paracrystals in the cytoplasm. These paracrystals are retained in cytoskeletons prepared by Triton extraction and are stable to treatment with calcium. The direct addition of vinblastine to cytoskeletons does not alter the organization of microtubules. The two effects of vinblastine are concentration-dependent, as assayed by binding of [3H]taxol and tubulin immunofluorescence. At low concentrations, vinblastine depolymerizes cellular microtubules; at high concentrations the drug induces the formation of paracrystals.

AB - Vinblastine has two distinct tubulin-related effects in 3T6 fibroblasts and J774.2 macrophages. It depolymerizes microtubules and it induces the formation of paracrystals in the cytoplasm. These paracrystals are retained in cytoskeletons prepared by Triton extraction and are stable to treatment with calcium. The direct addition of vinblastine to cytoskeletons does not alter the organization of microtubules. The two effects of vinblastine are concentration-dependent, as assayed by binding of [3H]taxol and tubulin immunofluorescence. At low concentrations, vinblastine depolymerizes cellular microtubules; at high concentrations the drug induces the formation of paracrystals.

UR - http://www.scopus.com/inward/record.url?scp=0021364749&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021364749&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(84)90715-8

DO - 10.1016/0014-4827(84)90715-8

M3 - Article

C2 - 6141062

AN - SCOPUS:0021364749

SN - 0014-4827

VL - 150

SP - 205

EP - 212

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 1

ER -

Vinblastine paracrystals from cultured cells are calcium-stable

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this