Vesicle fusion proteins in rat inner medullary collecting duct and amphibian bladder

N. Franki, Frank P. Macaluso, Y. Gao, R. M. Hays

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The delivery of water channels to the apical membrane in response to antidiuretic hormone (ADH) requires the targeting of channel-containing vesicles to specific sites in the membrane, followed by fusion and exocytosis. A complex array of proteins is now believed to mediate targeting and fusion in eukaryotic cells. They include N-ethylmaleimide-sensitive fusion protein (NSF), soluble NSF attachment proteins (SNAP), and cellubrevin, a vesicle-associated protein present in the nerve terminal. We asked whether these proteins are in epithelial cells of rat inner medullary collecting duct (IMCD) and amphibian bladder. Immunoblots on both tissues showed the presence of NSF and α-SNAP. Cellubrevin was present in immunoblots of the IMCD, but not the bladder. Immunogold electron microscopy showed NSF, α-SNAP, and cellubrevin in rat IMCD cells, with vesicular labeling. In the bladder, NSF was seen on vesicles and aggrephores. We conclude that components of the vesicle-targeting and fusion systems are present in kidney and amphibian bladder and may mediate a wide variety of fusion events, including those initiated by ADH.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume268
Issue number3 37-3
StatePublished - 1995

Fingerprint

N-Ethylmaleimide-Sensitive Proteins
Vesicle-Associated Membrane Protein 3
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
Amphibians
bladder
Ducts
amphibians
Rats
Urinary Bladder
Fusion reactions
rats
Vasopressins
Proteins
proteins
Membranes
Protein Array Analysis
Aquaporins
Membrane Fusion
Exocytosis
Eukaryotic Cells

Keywords

  • antidiuretic hormone
  • cellubrevin
  • N-ethylmaleimide-sensitive fusion protein
  • N-ethylmaleimide-sensitive fusion protein attachment proteins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

Vesicle fusion proteins in rat inner medullary collecting duct and amphibian bladder. / Franki, N.; Macaluso, Frank P.; Gao, Y.; Hays, R. M.

In: American Journal of Physiology - Cell Physiology, Vol. 268, No. 3 37-3, 1995.

Research output: Contribution to journalArticle

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