Ventricular dilation and elevated aqueductal pulsations in a new experimental model of communicating hydrocephalus

Mark E. Wagshul, J. P. McAllister, S. Rashid, J. Li, M. R. Egnor, M. L. Walker, M. Yu, S. D. Smith, G. Zhang, J. J. Chen, H. Benveniste

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In communicating hydrocephalus (CH), explanations for the symptoms and clear-cut effective treatments remain elusive. Pulsatile flow through the cerebral aqueduct is often significantly elevated, but a clear link between abnormal pulsations and ventriculomegaly has yet to be identified. We sought to demonstrate measurement of pulsatile aqueductal flow of CSF in the rat, and to characterize the temporal changes in CSF pulsations in a new model of CH. Hydrocephalus was induced by injection of kaolin into the basal cisterns of adult rats (n = 18). Ventricular volume and aqueductal pulsations were measured on a 9.4 T MRI over a one month period. Half of the animals developed ventricular dilation, with increased ventricular volume and pulsations as early as one day post-induction, and marked chronic elevations compared to intact controls (volume: 130.15 ± 83.21 μl vs. 15.52 ± 2.00 μl; pulsations: 114.51 nl ± 106.29 vs. 0.72 ± 0.13 nl). Similar to the clinical presentation, the relationship between ventricular size and pulsations was quite variable. However, the pulsation time-course revealed two distinct sub-types of hydrocephalic animals: those with markedly elevated pulsations which persisted over time, and those with mildly elevated pulsations which returned to near normal levels after one week. These groups were associated with severe and mild ventriculomegaly respectively. Thus, aqueductal flow can be measured in the rat using high-field MRI and basal cistern-induced CH is associated with an immediate change in CSF pulsatility. At the same time, our results highlight the complex nature of aqueductal pulsation and its relationship to ventricular dilation.

Original languageEnglish (US)
Pages (from-to)33-40
Number of pages8
JournalExperimental Neurology
Volume218
Issue number1
DOIs
StatePublished - Jul 2009
Externally publishedYes

Fingerprint

Hydrocephalus
Dilatation
Theoretical Models
Pulsatile Flow
Cerebral Aqueduct
Kaolin
Injections
Therapeutics

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Ventricular dilation and elevated aqueductal pulsations in a new experimental model of communicating hydrocephalus. / Wagshul, Mark E.; McAllister, J. P.; Rashid, S.; Li, J.; Egnor, M. R.; Walker, M. L.; Yu, M.; Smith, S. D.; Zhang, G.; Chen, J. J.; Benveniste, H.

In: Experimental Neurology, Vol. 218, No. 1, 07.2009, p. 33-40.

Research output: Contribution to journalArticle

Wagshul, ME, McAllister, JP, Rashid, S, Li, J, Egnor, MR, Walker, ML, Yu, M, Smith, SD, Zhang, G, Chen, JJ & Benveniste, H 2009, 'Ventricular dilation and elevated aqueductal pulsations in a new experimental model of communicating hydrocephalus', Experimental Neurology, vol. 218, no. 1, pp. 33-40. https://doi.org/10.1016/j.expneurol.2009.03.034
Wagshul, Mark E. ; McAllister, J. P. ; Rashid, S. ; Li, J. ; Egnor, M. R. ; Walker, M. L. ; Yu, M. ; Smith, S. D. ; Zhang, G. ; Chen, J. J. ; Benveniste, H. / Ventricular dilation and elevated aqueductal pulsations in a new experimental model of communicating hydrocephalus. In: Experimental Neurology. 2009 ; Vol. 218, No. 1. pp. 33-40.
@article{7e07fe1e870a4a9aa59c71c58fa84903,
title = "Ventricular dilation and elevated aqueductal pulsations in a new experimental model of communicating hydrocephalus",
abstract = "In communicating hydrocephalus (CH), explanations for the symptoms and clear-cut effective treatments remain elusive. Pulsatile flow through the cerebral aqueduct is often significantly elevated, but a clear link between abnormal pulsations and ventriculomegaly has yet to be identified. We sought to demonstrate measurement of pulsatile aqueductal flow of CSF in the rat, and to characterize the temporal changes in CSF pulsations in a new model of CH. Hydrocephalus was induced by injection of kaolin into the basal cisterns of adult rats (n = 18). Ventricular volume and aqueductal pulsations were measured on a 9.4 T MRI over a one month period. Half of the animals developed ventricular dilation, with increased ventricular volume and pulsations as early as one day post-induction, and marked chronic elevations compared to intact controls (volume: 130.15 ± 83.21 μl vs. 15.52 ± 2.00 μl; pulsations: 114.51 nl ± 106.29 vs. 0.72 ± 0.13 nl). Similar to the clinical presentation, the relationship between ventricular size and pulsations was quite variable. However, the pulsation time-course revealed two distinct sub-types of hydrocephalic animals: those with markedly elevated pulsations which persisted over time, and those with mildly elevated pulsations which returned to near normal levels after one week. These groups were associated with severe and mild ventriculomegaly respectively. Thus, aqueductal flow can be measured in the rat using high-field MRI and basal cistern-induced CH is associated with an immediate change in CSF pulsatility. At the same time, our results highlight the complex nature of aqueductal pulsation and its relationship to ventricular dilation.",
author = "Wagshul, {Mark E.} and McAllister, {J. P.} and S. Rashid and J. Li and Egnor, {M. R.} and Walker, {M. L.} and M. Yu and Smith, {S. D.} and G. Zhang and Chen, {J. J.} and H. Benveniste",
year = "2009",
month = "7",
doi = "10.1016/j.expneurol.2009.03.034",
language = "English (US)",
volume = "218",
pages = "33--40",
journal = "Experimental Neurology",
issn = "0014-4886",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Ventricular dilation and elevated aqueductal pulsations in a new experimental model of communicating hydrocephalus

AU - Wagshul, Mark E.

AU - McAllister, J. P.

AU - Rashid, S.

AU - Li, J.

AU - Egnor, M. R.

AU - Walker, M. L.

AU - Yu, M.

AU - Smith, S. D.

AU - Zhang, G.

AU - Chen, J. J.

AU - Benveniste, H.

PY - 2009/7

Y1 - 2009/7

N2 - In communicating hydrocephalus (CH), explanations for the symptoms and clear-cut effective treatments remain elusive. Pulsatile flow through the cerebral aqueduct is often significantly elevated, but a clear link between abnormal pulsations and ventriculomegaly has yet to be identified. We sought to demonstrate measurement of pulsatile aqueductal flow of CSF in the rat, and to characterize the temporal changes in CSF pulsations in a new model of CH. Hydrocephalus was induced by injection of kaolin into the basal cisterns of adult rats (n = 18). Ventricular volume and aqueductal pulsations were measured on a 9.4 T MRI over a one month period. Half of the animals developed ventricular dilation, with increased ventricular volume and pulsations as early as one day post-induction, and marked chronic elevations compared to intact controls (volume: 130.15 ± 83.21 μl vs. 15.52 ± 2.00 μl; pulsations: 114.51 nl ± 106.29 vs. 0.72 ± 0.13 nl). Similar to the clinical presentation, the relationship between ventricular size and pulsations was quite variable. However, the pulsation time-course revealed two distinct sub-types of hydrocephalic animals: those with markedly elevated pulsations which persisted over time, and those with mildly elevated pulsations which returned to near normal levels after one week. These groups were associated with severe and mild ventriculomegaly respectively. Thus, aqueductal flow can be measured in the rat using high-field MRI and basal cistern-induced CH is associated with an immediate change in CSF pulsatility. At the same time, our results highlight the complex nature of aqueductal pulsation and its relationship to ventricular dilation.

AB - In communicating hydrocephalus (CH), explanations for the symptoms and clear-cut effective treatments remain elusive. Pulsatile flow through the cerebral aqueduct is often significantly elevated, but a clear link between abnormal pulsations and ventriculomegaly has yet to be identified. We sought to demonstrate measurement of pulsatile aqueductal flow of CSF in the rat, and to characterize the temporal changes in CSF pulsations in a new model of CH. Hydrocephalus was induced by injection of kaolin into the basal cisterns of adult rats (n = 18). Ventricular volume and aqueductal pulsations were measured on a 9.4 T MRI over a one month period. Half of the animals developed ventricular dilation, with increased ventricular volume and pulsations as early as one day post-induction, and marked chronic elevations compared to intact controls (volume: 130.15 ± 83.21 μl vs. 15.52 ± 2.00 μl; pulsations: 114.51 nl ± 106.29 vs. 0.72 ± 0.13 nl). Similar to the clinical presentation, the relationship between ventricular size and pulsations was quite variable. However, the pulsation time-course revealed two distinct sub-types of hydrocephalic animals: those with markedly elevated pulsations which persisted over time, and those with mildly elevated pulsations which returned to near normal levels after one week. These groups were associated with severe and mild ventriculomegaly respectively. Thus, aqueductal flow can be measured in the rat using high-field MRI and basal cistern-induced CH is associated with an immediate change in CSF pulsatility. At the same time, our results highlight the complex nature of aqueductal pulsation and its relationship to ventricular dilation.

UR - http://www.scopus.com/inward/record.url?scp=67349194957&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67349194957&partnerID=8YFLogxK

U2 - 10.1016/j.expneurol.2009.03.034

DO - 10.1016/j.expneurol.2009.03.034

M3 - Article

C2 - 19348801

AN - SCOPUS:67349194957

VL - 218

SP - 33

EP - 40

JO - Experimental Neurology

JF - Experimental Neurology

SN - 0014-4886

IS - 1

ER -