Vasopressin decreases immunogold labeling of apical actin in the toad bladder granular cell

Y. Gao, N. Franki, Frank P. Macaluso, R. M. Hays

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Studies with the confocal microscope have shown that arginine vasopressin (AVP) depolymerizes F-actin in the apical region of the toad bladder granular cell. However, the resolution of the fluorescence microscope is not great enough to reveal the exact pattern of depolymerization or the relative extent to which microvillar and subapical membrane actin pools contribute to overall depolymerization. We have developed an electron microscopic immunogold method that shows a significant decrease in immunogold labeling of actin in the region just below the apical membrane, with the decrease most pronounced in regions adjacent to the microvilli. There was no significant change of immunogold labeling within the microvilli themselves. Our studies show a reorganization of the actin cytoskeleton in the region of the granular cell, where water channel-carrying vesicles are positioned and fuse in response to AVP.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume263
Issue number4 32-4
StatePublished - 1992

Fingerprint

Vasopressins
Anura
Labeling
Actins
Urinary Bladder
Arginine Vasopressin
Microvilli
Depolymerization
Aquaporins
Microscopes
Membranes
Actin Cytoskeleton
Electric fuses
Fluorescence
Electrons

Keywords

  • F-actin
  • water channels

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Vasopressin decreases immunogold labeling of apical actin in the toad bladder granular cell. / Gao, Y.; Franki, N.; Macaluso, Frank P.; Hays, R. M.

In: American Journal of Physiology - Cell Physiology, Vol. 263, No. 4 32-4, 1992.

Research output: Contribution to journalArticle

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