Variations in the composition of mammalian SWI/SNF chromatin remodelling complexes

Jessica Ryme, Patrik K.M. Asp, Stefanie Böhm, Erica Cavellán, Ann Kristin Östlund Farrants

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The ATP-dependent chromatin remodelling complexes SWI/SNF alter the chromatin structure in transcriptional regulation. Several classes of mammalian SWI/SNF complex have been isolated biochemically, distinguished by a few specific subunits, such as the BAF-specific BAF250A, BAF250B and BRM, and the PBAF-specific BAF180. We have determined the complex compositions using low stringency immunoprecipitation (IP) and shown that the pattern of subunit interactions was more diverse than previously defined classes had predicted. The subunit association at five gene promoters that depend on the SWI/SNF activity varied and the sequential chromatin immunoprecipitations revealed that different class-specific subunits occupied the promoters at the same time. The low-stringency IP showed that the BAF-specific BAF250A and BAF250B and the PBAF-specific BAF180 co-exist in a subset of SWI/SNF complexes, and fractionation of nuclear extract on size-exclusion chromatography demonstrated that sub-complexes with unorthodox subunit compositions were present in the cell. We propose a model in which the constellations of SWI/SNF complexes are "tailored" for each specific chromatin target and depend on the local chromatin environment to which complexes and sub-complexes are recruited.

Original languageEnglish (US)
Pages (from-to)565-576
Number of pages12
JournalJournal of Cellular Biochemistry
Volume108
Issue number3
DOIs
StatePublished - Oct 15 2009
Externally publishedYes

Fingerprint

Chromatin Assembly and Disassembly
Chromatin
Immunoprecipitation
Chemical analysis
Chromatin Immunoprecipitation
Gel Chromatography
Adenosine Triphosphate
Size exclusion chromatography
Fractionation
Genes
Association reactions

Keywords

  • BAF proteins
  • BRG1
  • BRM
  • Chromatin remodelling

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Variations in the composition of mammalian SWI/SNF chromatin remodelling complexes. / Ryme, Jessica; Asp, Patrik K.M.; Böhm, Stefanie; Cavellán, Erica; Farrants, Ann Kristin Östlund.

In: Journal of Cellular Biochemistry, Vol. 108, No. 3, 15.10.2009, p. 565-576.

Research output: Contribution to journalArticle

Ryme, Jessica ; Asp, Patrik K.M. ; Böhm, Stefanie ; Cavellán, Erica ; Farrants, Ann Kristin Östlund. / Variations in the composition of mammalian SWI/SNF chromatin remodelling complexes. In: Journal of Cellular Biochemistry. 2009 ; Vol. 108, No. 3. pp. 565-576.
@article{769d2aaedcdc44cb93100509b5acee77,
title = "Variations in the composition of mammalian SWI/SNF chromatin remodelling complexes",
abstract = "The ATP-dependent chromatin remodelling complexes SWI/SNF alter the chromatin structure in transcriptional regulation. Several classes of mammalian SWI/SNF complex have been isolated biochemically, distinguished by a few specific subunits, such as the BAF-specific BAF250A, BAF250B and BRM, and the PBAF-specific BAF180. We have determined the complex compositions using low stringency immunoprecipitation (IP) and shown that the pattern of subunit interactions was more diverse than previously defined classes had predicted. The subunit association at five gene promoters that depend on the SWI/SNF activity varied and the sequential chromatin immunoprecipitations revealed that different class-specific subunits occupied the promoters at the same time. The low-stringency IP showed that the BAF-specific BAF250A and BAF250B and the PBAF-specific BAF180 co-exist in a subset of SWI/SNF complexes, and fractionation of nuclear extract on size-exclusion chromatography demonstrated that sub-complexes with unorthodox subunit compositions were present in the cell. We propose a model in which the constellations of SWI/SNF complexes are {"}tailored{"} for each specific chromatin target and depend on the local chromatin environment to which complexes and sub-complexes are recruited.",
keywords = "BAF proteins, BRG1, BRM, Chromatin remodelling",
author = "Jessica Ryme and Asp, {Patrik K.M.} and Stefanie B{\"o}hm and Erica Cavell{\'a}n and Farrants, {Ann Kristin {\"O}stlund}",
year = "2009",
month = "10",
day = "15",
doi = "10.1002/jcb.22288",
language = "English (US)",
volume = "108",
pages = "565--576",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Variations in the composition of mammalian SWI/SNF chromatin remodelling complexes

AU - Ryme, Jessica

AU - Asp, Patrik K.M.

AU - Böhm, Stefanie

AU - Cavellán, Erica

AU - Farrants, Ann Kristin Östlund

PY - 2009/10/15

Y1 - 2009/10/15

N2 - The ATP-dependent chromatin remodelling complexes SWI/SNF alter the chromatin structure in transcriptional regulation. Several classes of mammalian SWI/SNF complex have been isolated biochemically, distinguished by a few specific subunits, such as the BAF-specific BAF250A, BAF250B and BRM, and the PBAF-specific BAF180. We have determined the complex compositions using low stringency immunoprecipitation (IP) and shown that the pattern of subunit interactions was more diverse than previously defined classes had predicted. The subunit association at five gene promoters that depend on the SWI/SNF activity varied and the sequential chromatin immunoprecipitations revealed that different class-specific subunits occupied the promoters at the same time. The low-stringency IP showed that the BAF-specific BAF250A and BAF250B and the PBAF-specific BAF180 co-exist in a subset of SWI/SNF complexes, and fractionation of nuclear extract on size-exclusion chromatography demonstrated that sub-complexes with unorthodox subunit compositions were present in the cell. We propose a model in which the constellations of SWI/SNF complexes are "tailored" for each specific chromatin target and depend on the local chromatin environment to which complexes and sub-complexes are recruited.

AB - The ATP-dependent chromatin remodelling complexes SWI/SNF alter the chromatin structure in transcriptional regulation. Several classes of mammalian SWI/SNF complex have been isolated biochemically, distinguished by a few specific subunits, such as the BAF-specific BAF250A, BAF250B and BRM, and the PBAF-specific BAF180. We have determined the complex compositions using low stringency immunoprecipitation (IP) and shown that the pattern of subunit interactions was more diverse than previously defined classes had predicted. The subunit association at five gene promoters that depend on the SWI/SNF activity varied and the sequential chromatin immunoprecipitations revealed that different class-specific subunits occupied the promoters at the same time. The low-stringency IP showed that the BAF-specific BAF250A and BAF250B and the PBAF-specific BAF180 co-exist in a subset of SWI/SNF complexes, and fractionation of nuclear extract on size-exclusion chromatography demonstrated that sub-complexes with unorthodox subunit compositions were present in the cell. We propose a model in which the constellations of SWI/SNF complexes are "tailored" for each specific chromatin target and depend on the local chromatin environment to which complexes and sub-complexes are recruited.

KW - BAF proteins

KW - BRG1

KW - BRM

KW - Chromatin remodelling

UR - http://www.scopus.com/inward/record.url?scp=70350153633&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350153633&partnerID=8YFLogxK

U2 - 10.1002/jcb.22288

DO - 10.1002/jcb.22288

M3 - Article

C2 - 19650111

AN - SCOPUS:70350153633

VL - 108

SP - 565

EP - 576

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 3

ER -