Variation in restriction fragment length and methylation pattern of rat MHC class I genes

A. G. Uitterlinden, Jan Vijg, M. J. Giphart, D. L. Knook

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Rat MHC (RT.1) class I genes were analyzed by performing Southern blot analysis of digests of genomic DNA isolated from three inbred rat strains using a human derived HLA cDNA probe specific for class I genes. A large number of hybridizing restriction fragments indicated that the rat MHC is a large multigene family. Polymorphic class I DNA restriction fragments specific for the different haplotypes were found for three restriction enzymes used, indicating a high degree of restriction fragment length polymorphism of RT.1 class I genes. Analysis of the methylation state of class I RT.1 genes showed that they are hypermethylated to an extent that is haplotype specific. Furthermore, variation in number and size of Hpa II fragments were observed when comparing liver and spleen DNA from two rat strains sharing the same haplotype. This indicates methylation variation between these organs for RT.1 class I genes.

Original languageEnglish (US)
Pages (from-to)215-222
Number of pages8
JournalExperimental and Clinical Immunogenetics
Volume2
Issue number4
StatePublished - 1985
Externally publishedYes

Fingerprint

MHC Class I Genes
Methylation
Haplotypes
DNA
Inbred Strains Rats
Multigene Family
Southern Blotting
Restriction Fragment Length Polymorphisms
Spleen
Complementary DNA
Liver
Enzymes
Genes

ASJC Scopus subject areas

  • Genetics
  • Immunology
  • Genetics(clinical)

Cite this

Variation in restriction fragment length and methylation pattern of rat MHC class I genes. / Uitterlinden, A. G.; Vijg, Jan; Giphart, M. J.; Knook, D. L.

In: Experimental and Clinical Immunogenetics, Vol. 2, No. 4, 1985, p. 215-222.

Research output: Contribution to journalArticle

Uitterlinden, A. G. ; Vijg, Jan ; Giphart, M. J. ; Knook, D. L. / Variation in restriction fragment length and methylation pattern of rat MHC class I genes. In: Experimental and Clinical Immunogenetics. 1985 ; Vol. 2, No. 4. pp. 215-222.
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