Abstract
Cells of the MPC 11 mouse myeloma cell line, which produces an IgG(2b) immunoglobulin, were subjected to mutagenesis with Melphalan or with ICR 191, after which they were cloned in soft agar. Approximately 0.5% of the clones produced altered heavy chains that: (i) were the same size as or larger than the parent; (ii) no longer were recognized by antibody specific for the parent γ2b subclass; (iii) were recognized by antibody specific for the γ2a subclass; (iv) were recognized by antibody against the Fab (NH2 terminal half) of the parental immunoglobulin, but lacked some of the antigenic determinants of the Fc (COOH terminal half) of the heavy chain; (v) lacked many of the tryptic/chymotryptic peptides found in the parent; (vi) contained tryptic/chymotryptic peptides that were not present in the parent but were present in an unrelated γ2a myeloma heavy chain; and (vii) assembled with light chains by a pathway typical of IgG(2a) myelomas.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1427-1430 |
| Number of pages | 4 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 72 |
| Issue number | 4 |
| State | Published - 1975 |
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ASJC Scopus subject areas
- General
- Genetics
Cite this
Variants of a mouse myeloma cell line that synthesize immunoglobulin heavy chains having an altered serotype. / Preud'homme, J. L.; Birshtein, B. K.; Scharff, Matthew D.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, No. 4, 1975, p. 1427-1430.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Variants of a mouse myeloma cell line that synthesize immunoglobulin heavy chains having an altered serotype
AU - Preud'homme, J. L.
AU - Birshtein, B. K.
AU - Scharff, Matthew D.
PY - 1975
Y1 - 1975
N2 - Cells of the MPC 11 mouse myeloma cell line, which produces an IgG(2b) immunoglobulin, were subjected to mutagenesis with Melphalan or with ICR 191, after which they were cloned in soft agar. Approximately 0.5% of the clones produced altered heavy chains that: (i) were the same size as or larger than the parent; (ii) no longer were recognized by antibody specific for the parent γ2b subclass; (iii) were recognized by antibody specific for the γ2a subclass; (iv) were recognized by antibody against the Fab (NH2 terminal half) of the parental immunoglobulin, but lacked some of the antigenic determinants of the Fc (COOH terminal half) of the heavy chain; (v) lacked many of the tryptic/chymotryptic peptides found in the parent; (vi) contained tryptic/chymotryptic peptides that were not present in the parent but were present in an unrelated γ2a myeloma heavy chain; and (vii) assembled with light chains by a pathway typical of IgG(2a) myelomas.
AB - Cells of the MPC 11 mouse myeloma cell line, which produces an IgG(2b) immunoglobulin, were subjected to mutagenesis with Melphalan or with ICR 191, after which they were cloned in soft agar. Approximately 0.5% of the clones produced altered heavy chains that: (i) were the same size as or larger than the parent; (ii) no longer were recognized by antibody specific for the parent γ2b subclass; (iii) were recognized by antibody specific for the γ2a subclass; (iv) were recognized by antibody against the Fab (NH2 terminal half) of the parental immunoglobulin, but lacked some of the antigenic determinants of the Fc (COOH terminal half) of the heavy chain; (v) lacked many of the tryptic/chymotryptic peptides found in the parent; (vi) contained tryptic/chymotryptic peptides that were not present in the parent but were present in an unrelated γ2a myeloma heavy chain; and (vii) assembled with light chains by a pathway typical of IgG(2a) myelomas.
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UR - http://www.scopus.com/inward/citedby.url?scp=0016694067&partnerID=8YFLogxK
M3 - Article
C2 - 805428
AN - SCOPUS:0016694067
VL - 72
SP - 1427
EP - 1430
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 4
ER -