Vanadate stimulates adenylate cyclase via the guanine nucleotide regulatory protein by a mechanism differing from that of fluoride

W. Krawietz, R. W. Downs, Allen M. Spiegel, G. D. Aurbach

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Vanadate stimulates adenylate cyclase activity in turkey erythrocyte membranes. The maximal stimulation is 7-fold over basal at 3 mM vanadate; higher concentrations are inhibitory. A suboptimal concentration of fluoride (1 mM) together with vanadate (3 mM) activates adenylate cyclase in a non-additive manner; cyclase activation by optimal fluoride (10 mM) is inhibited by vanadate (3 mM). There is no stimulation by vanadate of adenylate cyclase activity (measured either with Mg2+ or Mn2+) in CYC- S49 lymphoma cell membranes. Vanadate (3 mM) shows no effect on binding of β-adrenergic agonists or antagonists to the [3H](-)-dihydroalprenolol binding site in turkey erythrocyte membranes. These results suggest that the effect of vanadate on adenylate cyclase is mediated through the nucleotide regulatory protein and may act by a mechanism similar to fluoride. However, in cholera toxin-treated membranes as well as in GDP-β-S plus isoproterenol-treated membranes, fluoride-stimulated adenylate cyclase activity is significantly reduced, but vanadate stimulation is not. Our results suggest that although the actions of vanadate and fluoride in adenylate cyclase may each involve the nucleotide regulatory unit, the exact mechanisms of activation by the two anions differ.

Original languageEnglish (US)
Pages (from-to)843-848
Number of pages6
JournalBiochemical Pharmacology
Volume31
Issue number5
DOIs
StatePublished - Mar 1 1982
Externally publishedYes

Fingerprint

Vanadates
Fluorides
GTP-Binding Proteins
Adenylyl Cyclases
Membranes
Erythrocyte Membrane
Nucleotides
Chemical activation
Dihydroalprenolol
Adrenergic Agonists
Adrenergic Antagonists
Cholera Toxin
Cell membranes
Isoproterenol
Anions
Lymphoma
Binding Sites
Cell Membrane

ASJC Scopus subject areas

  • Pharmacology

Cite this

Vanadate stimulates adenylate cyclase via the guanine nucleotide regulatory protein by a mechanism differing from that of fluoride. / Krawietz, W.; Downs, R. W.; Spiegel, Allen M.; Aurbach, G. D.

In: Biochemical Pharmacology, Vol. 31, No. 5, 01.03.1982, p. 843-848.

Research output: Contribution to journalArticle

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