Utilization of spectrins βI and βIII in diagnosis of hepatocellular carcinoma

Shaomin Hu, Deborah Jue, Joseph Albanese, Yanhua Wang, Qiang Liu

Research output: Contribution to journalArticle

Abstract

Spectrins are a group of cytoskeletal proteins which participate in many important cellular functions. It has been suggested that loss of spectrin isoforms may be associated with tumorigenesis of lymphoma, leukemia, gastric cancer and hepatocellular carcinoma (HCC). We recently reported that βI spectrin expression was present in normal hepatocytes but lost in HCC cells, which suggested that spectrins may be helpful markers in diagnosis of HCC. In this study, using immunohistochemical staining, we further investigated the expression pattern of four spectrin isoforms (αII, βI-III) on different benign and malignant liver tumors including focal nodular hyperplasia (FNH), hepatic adenoma (HA), HCC, and cholangiocarcinoma (CC). The results revealed that βI spectrin was moderately to strongly positive in FNH and HA tissues, but was only weakly positive or lost in HCC cases and was weakly positive in all CC cases. In addition, the βIII spectrin, majority of which was moderately positive in both FNH and HA tissues, was mostly lost in poorly differentiated HCC but remained at least moderately positive in most CC cases. These results suggest that spectrins βI and βIII may be used to differentiate well differentiated HCC from FNH or HA, and poorly differentiated HCC from CC, respectively.

Original languageEnglish (US)
Pages (from-to)86-91
Number of pages6
JournalAnnals of Diagnostic Pathology
Volume39
DOIs
StatePublished - Apr 1 2019

Fingerprint

Spectrin
Hepatocellular Carcinoma
Focal Nodular Hyperplasia
Cholangiocarcinoma
Adenoma
Liver
Protein Isoforms
Cytoskeletal Proteins
Stomach Neoplasms
Hepatocytes
Lymphoma
Carcinogenesis
Leukemia
Staining and Labeling

Keywords

  • Cholangiocarcinoma
  • Focal nodular hyperplasia
  • Hepatic adenoma
  • Hepatocellular carcinoma
  • Spectrin βI
  • Spectrin βIII

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Utilization of spectrins βI and βIII in diagnosis of hepatocellular carcinoma. / Hu, Shaomin; Jue, Deborah; Albanese, Joseph; Wang, Yanhua; Liu, Qiang.

In: Annals of Diagnostic Pathology, Vol. 39, 01.04.2019, p. 86-91.

Research output: Contribution to journalArticle

@article{1f35a773a4b74a2cbd6797b334c28c79,
title = "Utilization of spectrins βI and βIII in diagnosis of hepatocellular carcinoma",
abstract = "Spectrins are a group of cytoskeletal proteins which participate in many important cellular functions. It has been suggested that loss of spectrin isoforms may be associated with tumorigenesis of lymphoma, leukemia, gastric cancer and hepatocellular carcinoma (HCC). We recently reported that βI spectrin expression was present in normal hepatocytes but lost in HCC cells, which suggested that spectrins may be helpful markers in diagnosis of HCC. In this study, using immunohistochemical staining, we further investigated the expression pattern of four spectrin isoforms (αII, βI-III) on different benign and malignant liver tumors including focal nodular hyperplasia (FNH), hepatic adenoma (HA), HCC, and cholangiocarcinoma (CC). The results revealed that βI spectrin was moderately to strongly positive in FNH and HA tissues, but was only weakly positive or lost in HCC cases and was weakly positive in all CC cases. In addition, the βIII spectrin, majority of which was moderately positive in both FNH and HA tissues, was mostly lost in poorly differentiated HCC but remained at least moderately positive in most CC cases. These results suggest that spectrins βI and βIII may be used to differentiate well differentiated HCC from FNH or HA, and poorly differentiated HCC from CC, respectively.",
keywords = "Cholangiocarcinoma, Focal nodular hyperplasia, Hepatic adenoma, Hepatocellular carcinoma, Spectrin βI, Spectrin βIII",
author = "Shaomin Hu and Deborah Jue and Joseph Albanese and Yanhua Wang and Qiang Liu",
year = "2019",
month = "4",
day = "1",
doi = "10.1016/j.anndiagpath.2019.02.009",
language = "English (US)",
volume = "39",
pages = "86--91",
journal = "Annals of Diagnostic Pathology",
issn = "1092-9134",
publisher = "W.B. Saunders Ltd",

}

TY - JOUR

T1 - Utilization of spectrins βI and βIII in diagnosis of hepatocellular carcinoma

AU - Hu, Shaomin

AU - Jue, Deborah

AU - Albanese, Joseph

AU - Wang, Yanhua

AU - Liu, Qiang

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Spectrins are a group of cytoskeletal proteins which participate in many important cellular functions. It has been suggested that loss of spectrin isoforms may be associated with tumorigenesis of lymphoma, leukemia, gastric cancer and hepatocellular carcinoma (HCC). We recently reported that βI spectrin expression was present in normal hepatocytes but lost in HCC cells, which suggested that spectrins may be helpful markers in diagnosis of HCC. In this study, using immunohistochemical staining, we further investigated the expression pattern of four spectrin isoforms (αII, βI-III) on different benign and malignant liver tumors including focal nodular hyperplasia (FNH), hepatic adenoma (HA), HCC, and cholangiocarcinoma (CC). The results revealed that βI spectrin was moderately to strongly positive in FNH and HA tissues, but was only weakly positive or lost in HCC cases and was weakly positive in all CC cases. In addition, the βIII spectrin, majority of which was moderately positive in both FNH and HA tissues, was mostly lost in poorly differentiated HCC but remained at least moderately positive in most CC cases. These results suggest that spectrins βI and βIII may be used to differentiate well differentiated HCC from FNH or HA, and poorly differentiated HCC from CC, respectively.

AB - Spectrins are a group of cytoskeletal proteins which participate in many important cellular functions. It has been suggested that loss of spectrin isoforms may be associated with tumorigenesis of lymphoma, leukemia, gastric cancer and hepatocellular carcinoma (HCC). We recently reported that βI spectrin expression was present in normal hepatocytes but lost in HCC cells, which suggested that spectrins may be helpful markers in diagnosis of HCC. In this study, using immunohistochemical staining, we further investigated the expression pattern of four spectrin isoforms (αII, βI-III) on different benign and malignant liver tumors including focal nodular hyperplasia (FNH), hepatic adenoma (HA), HCC, and cholangiocarcinoma (CC). The results revealed that βI spectrin was moderately to strongly positive in FNH and HA tissues, but was only weakly positive or lost in HCC cases and was weakly positive in all CC cases. In addition, the βIII spectrin, majority of which was moderately positive in both FNH and HA tissues, was mostly lost in poorly differentiated HCC but remained at least moderately positive in most CC cases. These results suggest that spectrins βI and βIII may be used to differentiate well differentiated HCC from FNH or HA, and poorly differentiated HCC from CC, respectively.

KW - Cholangiocarcinoma

KW - Focal nodular hyperplasia

KW - Hepatic adenoma

KW - Hepatocellular carcinoma

KW - Spectrin βI

KW - Spectrin βIII

UR - http://www.scopus.com/inward/record.url?scp=85061825551&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061825551&partnerID=8YFLogxK

U2 - 10.1016/j.anndiagpath.2019.02.009

DO - 10.1016/j.anndiagpath.2019.02.009

M3 - Article

VL - 39

SP - 86

EP - 91

JO - Annals of Diagnostic Pathology

JF - Annals of Diagnostic Pathology

SN - 1092-9134

ER -