Using kinetic studies to uncover new Rb functions in inhibiting cell cycle progression

Peng Ji, Liang Zhu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A well-established biological activity of the tumor suppressor Rb is blocking G1/S cell cycle progression when reintroduced into cultured Rb-deficient tumor cells. The best understood molecular mechanism underlying this function is that Rb binds the transcription factor E2F to repress expression of S phase genes such as cyclins E and A. A recent kinetic study of this model further revealed that Rb represses Skp2 to stabilize p27, which inhibits the kinase activity associated with cyclins E and A before the decline in their protein levels, to arrest the cell cycle. This p27-stabilizing function of Rb is retained in a clinical partial penetrance Rb mutant that is biochemically inactive for E2F repression, suggesting a mechanism for Rb-mediated inhibition of tumor progression.

Original languageEnglish (US)
Pages (from-to)373-375
Number of pages3
JournalCell Cycle
Volume4
Issue number3
StatePublished - Mar 2005

Fingerprint

Tumors
Cyclin A
Cyclin E
Cell Cycle
Cells
Kinetics
E2F Transcription Factors
Neoplasms
Penetrance
Cell Cycle Checkpoints
Bioactivity
S Phase
Phosphotransferases
Genes
Proteins

Keywords

  • Cell cycle
  • E2F
  • Kinetic studies
  • p27
  • Partial penetrance
  • Retinoblastoma protein Rb
  • Skp2
  • Tumor progression
  • Tumor suppressor

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Cite this

Using kinetic studies to uncover new Rb functions in inhibiting cell cycle progression. / Ji, Peng; Zhu, Liang.

In: Cell Cycle, Vol. 4, No. 3, 03.2005, p. 373-375.

Research output: Contribution to journalArticle

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