Using kinetic studies to uncover new Rb functions in inhibiting cell cycle progression

Peng Ji, Liang Zhu

Research output: Contribution to journalShort survey

12 Scopus citations

Abstract

A well-established biological activity of the tumor suppressor Rb is blocking G1/S cell cycle progression when reintroduced into cultured Rb-deficient tumor cells. The best understood molecular mechanism underlying this function is that Rb binds the transcription factor E2F to repress expression of S phase genes such as cyclins E and A. A recent kinetic study of this model further revealed that Rb represses Skp2 to stabilize p27, which inhibits the kinase activity associated with cyclins E and A before the decline in their protein levels, to arrest the cell cycle. This p27-stabilizing function of Rb is retained in a clinical partial penetrance Rb mutant that is biochemically inactive for E2F repression, suggesting a mechanism for Rb-mediated inhibition of tumor progression.

Original languageEnglish (US)
Pages (from-to)373-375
Number of pages3
JournalCell Cycle
Volume4
Issue number3
DOIs
Publication statusPublished - Mar 2005

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Keywords

  • Cell cycle
  • E2F
  • Kinetic studies
  • Partial penetrance
  • Retinoblastoma protein Rb
  • Skp2
  • Tumor progression
  • Tumor suppressor
  • p27

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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