Use of single molecule fluorescence polarization microscopy to study protein conformation and dynamics of kinesin–microtubule complexes

Matthieu P.M.H. Benoit, Hernando J. Sosa

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Single molecule fluorescence polarization microscopy (smFPM) is a technique that enables to monitor changes in the orientation of a single labeled protein domain. Here we describe a smFPM microscope set-up and protocols to investigate conformational changes associated with the movement of motor proteins along cytoskeletal tracks.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages199-216
Number of pages18
Volume1665
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1665
ISSN (Print)1064-3745

Fingerprint

Polarization Microscopy
Protein Conformation
Fluorescence Polarization
Fluorescence Microscopy
Cytoskeletal Proteins
Single Molecule Imaging
Protein Domains

Keywords

  • BSR
  • Dichroism
  • Fluorescence polarization
  • Kinesin
  • Microtubule
  • Molecular motor
  • Single molecule

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Benoit, M. P. M. H., & Sosa, H. J. (2018). Use of single molecule fluorescence polarization microscopy to study protein conformation and dynamics of kinesin–microtubule complexes. In Methods in Molecular Biology (Vol. 1665, pp. 199-216). (Methods in Molecular Biology; Vol. 1665). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7271-5_11

Use of single molecule fluorescence polarization microscopy to study protein conformation and dynamics of kinesin–microtubule complexes. / Benoit, Matthieu P.M.H.; Sosa, Hernando J.

Methods in Molecular Biology. Vol. 1665 Humana Press Inc., 2018. p. 199-216 (Methods in Molecular Biology; Vol. 1665).

Research output: Chapter in Book/Report/Conference proceedingChapter

Benoit, MPMH & Sosa, HJ 2018, Use of single molecule fluorescence polarization microscopy to study protein conformation and dynamics of kinesin–microtubule complexes. in Methods in Molecular Biology. vol. 1665, Methods in Molecular Biology, vol. 1665, Humana Press Inc., pp. 199-216. https://doi.org/10.1007/978-1-4939-7271-5_11
Benoit MPMH, Sosa HJ. Use of single molecule fluorescence polarization microscopy to study protein conformation and dynamics of kinesin–microtubule complexes. In Methods in Molecular Biology. Vol. 1665. Humana Press Inc. 2018. p. 199-216. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7271-5_11
Benoit, Matthieu P.M.H. ; Sosa, Hernando J. / Use of single molecule fluorescence polarization microscopy to study protein conformation and dynamics of kinesin–microtubule complexes. Methods in Molecular Biology. Vol. 1665 Humana Press Inc., 2018. pp. 199-216 (Methods in Molecular Biology).
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