Use of antibodies to probe membrane glycoproteins associated with drug-resistant J774.2 cells

Ron Zeheb, Howard F. Beittenmiller, Susan Band Horwitz

Research output: Contribution to journalArticle

19 Scopus citations


Three drug-resistant sublines of the murine macrophage-like cell line J774.2 were selected in vitro for their ability to grow in high concentrations of either taxol, vinblastine, or colchicine. Each contains a major plasma membrane glycoprotein (130-150 kDa), which is barely seen in the drug-sensitive parental cell line. Polyclonal antibodies, raised against the glycoproteins present in the colchicine- and vinblastine-resistant cells, were used to probe for relationships among the three glycoproteins. Our observations suggest that the glycoproteins from the different drug-resistant cell lines share many common domains but are not identical.

Original languageEnglish (US)
Pages (from-to)732-739
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - Mar 13 1987


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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