Use of antibodies to probe membrane glycoproteins associated with drug-resistant J774.2 cells

Ron Zeheb, Howard F. Beittenmiller, Susan Band Horwitz

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Three drug-resistant sublines of the murine macrophage-like cell line J774.2 were selected in vitro for their ability to grow in high concentrations of either taxol, vinblastine, or colchicine. Each contains a major plasma membrane glycoprotein (130-150 kDa), which is barely seen in the drug-sensitive parental cell line. Polyclonal antibodies, raised against the glycoproteins present in the colchicine- and vinblastine-resistant cells, were used to probe for relationships among the three glycoproteins. Our observations suggest that the glycoproteins from the different drug-resistant cell lines share many common domains but are not identical.

Original languageEnglish (US)
Pages (from-to)732-739
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume143
Issue number2
DOIs
StatePublished - Mar 13 1987

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Membrane Glycoproteins
Glycoproteins
Vinblastine
Cells
Colchicine
Cell Line
Antibodies
Pharmaceutical Preparations
Macrophages
Cell membranes
Paclitaxel
Cell Membrane

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Use of antibodies to probe membrane glycoproteins associated with drug-resistant J774.2 cells. / Zeheb, Ron; Beittenmiller, Howard F.; Band Horwitz, Susan.

In: Biochemical and Biophysical Research Communications, Vol. 143, No. 2, 13.03.1987, p. 732-739.

Research output: Contribution to journalArticle

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