Urea-induced ROS generation causes insulin resistance in mice with chronic renal failure

Maria D'Apolito, Xue-Liang Du, Haihong Zong, Alessandra Catucci, Luigi Maiuri, Tiziana Trivisano, Massimo Pettoello-Mantovani, Angelo Campanozzi, Valeria Raia, Jeffrey E. Pessin, Michael Brownlee, Ida Giardino

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Although supraphysiological concentrations of urea are known to increase oxidative stress in cultured cells, it is generally thought that the elevated levels of urea in chronic renal failure patients have negligible toxicity. We previously demonstrated that ROS increase intracellular protein modification by O-linked β-N-acetyl-glucosamine (O-GlcNAc), and others showed that increased modification of insulin signaling molecules by O-GlcNAc reduces insulin signal transduction. Because both oxidative stress and insulin resistance have been observed in patients with end-stage renal disease, we sought to determine the role of urea in these phenotypes. Treatment of 3T3-L1 adipocytes with urea at disease-relevant concentrations induced ROS production, caused insulin resistance, increased expression of adipokines retinol binding protein 4 (RBP4) and resistin, and increased O-GlcNAc-modified insulin signaling molecules. Investigation of a mouse model of surgically induced renal failure (uremic mice) revealed increased ROS production, modification of insulin signaling molecules by O-GlcNAc, and increased expression of RBP4 and resistin in visceral adipose tissue. Uremic mice also displayed insulin resistance and glucose intolerance, and treatment with an antioxidant SOD/catalase mimetic normalized these defects. The SOD/catalase mimetic treatment also prevented the development of insulin resistance in normal mice after urea infusion. These data suggest that therapeutic targeting of urea-induced ROS may help reduce the high morbidity and mortality caused by end-stage renal disease.

Original languageEnglish (US)
Pages (from-to)203-213
Number of pages11
JournalJournal of Clinical Investigation
Volume120
Issue number1
DOIs
StatePublished - Jan 4 2010

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Chronic Kidney Failure
Insulin Resistance
Urea
Insulin
Resistin
Retinol-Binding Proteins
Catalase
Oxidative Stress
Adipokines
Glucose Intolerance
Intra-Abdominal Fat
Glucosamine
Therapeutics
Adipocytes
Renal Insufficiency
Cultured Cells
Signal Transduction
Antioxidants
Morbidity
Phenotype

ASJC Scopus subject areas

  • Medicine(all)

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Urea-induced ROS generation causes insulin resistance in mice with chronic renal failure. / D'Apolito, Maria; Du, Xue-Liang; Zong, Haihong; Catucci, Alessandra; Maiuri, Luigi; Trivisano, Tiziana; Pettoello-Mantovani, Massimo; Campanozzi, Angelo; Raia, Valeria; Pessin, Jeffrey E.; Brownlee, Michael; Giardino, Ida.

In: Journal of Clinical Investigation, Vol. 120, No. 1, 04.01.2010, p. 203-213.

Research output: Contribution to journalArticle

D'Apolito, M, Du, X-L, Zong, H, Catucci, A, Maiuri, L, Trivisano, T, Pettoello-Mantovani, M, Campanozzi, A, Raia, V, Pessin, JE, Brownlee, M & Giardino, I 2010, 'Urea-induced ROS generation causes insulin resistance in mice with chronic renal failure', Journal of Clinical Investigation, vol. 120, no. 1, pp. 203-213. https://doi.org/10.1172/JCI37672
D'Apolito, Maria ; Du, Xue-Liang ; Zong, Haihong ; Catucci, Alessandra ; Maiuri, Luigi ; Trivisano, Tiziana ; Pettoello-Mantovani, Massimo ; Campanozzi, Angelo ; Raia, Valeria ; Pessin, Jeffrey E. ; Brownlee, Michael ; Giardino, Ida. / Urea-induced ROS generation causes insulin resistance in mice with chronic renal failure. In: Journal of Clinical Investigation. 2010 ; Vol. 120, No. 1. pp. 203-213.
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