TY - JOUR
T1 - Unraveling neuronal dopamine transporter mechanisms with rotating disk electrode voltammetry
AU - Schenk, James O.
AU - Wright, Cortney
AU - Bjorklund, Nicole
N1 - Funding Information:
Support for this work to J.O.S. from NIDA grants RO1 DA07384, KO2 DA00184, and the Washington Alcohol and Drug Abuse Program (Legislative Initiative 171) is gratefully acknowledged.
PY - 2005/4/15
Y1 - 2005/4/15
N2 - Herein we describe how the rotating disk electrode voltammetric technique can be used to examine the mechanism(s) of the inward transport of dopamine by the neuronal transporter for dopamine (DAT). The usefulness of making measurements kinetically resolving dopamine transport, interpretations of changes in Km and Vmax, approaches to defining pre-steady-state binding of dopamine to DAT, interactions between competing inhibitors, chemical modification of functional groups within DAT, and a presentation of a hypothetical multi-state model of dopamine transport are presented and discussed.
AB - Herein we describe how the rotating disk electrode voltammetric technique can be used to examine the mechanism(s) of the inward transport of dopamine by the neuronal transporter for dopamine (DAT). The usefulness of making measurements kinetically resolving dopamine transport, interpretations of changes in Km and Vmax, approaches to defining pre-steady-state binding of dopamine to DAT, interactions between competing inhibitors, chemical modification of functional groups within DAT, and a presentation of a hypothetical multi-state model of dopamine transport are presented and discussed.
KW - Amphetamine
KW - Cocaine
KW - Transporter mechanism
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U2 - 10.1016/j.jneumeth.2004.09.021
DO - 10.1016/j.jneumeth.2004.09.021
M3 - Article
C2 - 15763135
AN - SCOPUS:14844300870
SN - 0165-0270
VL - 143
SP - 41
EP - 47
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 1
ER -