Unraveling neuronal dopamine transporter mechanisms with rotating disk electrode voltammetry

James O. Schenk, Cortney Wright, Nicole Bjorklund

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Herein we describe how the rotating disk electrode voltammetric technique can be used to examine the mechanism(s) of the inward transport of dopamine by the neuronal transporter for dopamine (DAT). The usefulness of making measurements kinetically resolving dopamine transport, interpretations of changes in Km and Vmax, approaches to defining pre-steady-state binding of dopamine to DAT, interactions between competing inhibitors, chemical modification of functional groups within DAT, and a presentation of a hypothetical multi-state model of dopamine transport are presented and discussed.

Original languageEnglish (US)
Pages (from-to)41-47
Number of pages7
JournalJournal of Neuroscience Methods
Volume143
Issue number1
DOIs
StatePublished - Apr 15 2005

Keywords

  • Amphetamine
  • Cocaine
  • Transporter mechanism

ASJC Scopus subject areas

  • Neuroscience(all)

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