Tyrosine phosphorylation of a common 57-kDa protein in growth factor-stimulated and -transformed cells

Wei Li, Y. G. Yeung, E. Richard Stanley

Research output: Contribution to journalArticle

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Abstract

Protein tyrosine phosphorylation was studied in macrophages and fibroblasts to identify putative components of post-receptor mitogenic pathways that might be functionally conserved in different cell types. Nondenaturing conditions were established for the approximately quantitative recovery of anti-phosphotyrosine antibody (αPY)-reactive proteins from cells. A common, 57-kDa αPY-reactive protein was identified by V8 protease peptide mapping in colony-stimulating factor-1 (CSF-1)- or granulocyte-macrophage colony-stimulating factor (GM-CSF)-stimulated BAC1.2F5 macrophages, in platelet-derived growth factor-stimulated NIH-3T3 cells, and in CSF-1-stimulated NIH-3T3 cells expressing the c-fms/CSF-1 receptor. The 57-kDa protein was phosphorylated on serine and tyrosine and was the only αPY-reactive protein band whose phosphorylation was reproducibly increased in GM-CSF-stimulated cells. The effect of the growth factors on the tyrosine phosphorylation of the 57-kDa protein could be mimicked by treatment of the cells with orthovanadate, a phosphotyrosine protein phosphatase inhibitor. In the absence of growth factors, tyrosine phosphorylation of the 57-kDa protein was higher in v-fms or c-fms (F969, S301)-transformed NIH-3T3 cells than in untransformed NIH-3T3 (c-fms) and NIH-3T3 (c-fms, F969) cells. These data indicate that the 57-kDa protein is a common target for growth factor-stimulated tyrosine phosphorylation and potentially important for growth factor mitogenic signaling.

Original languageEnglish (US)
Pages (from-to)6808-6814
Number of pages7
JournalJournal of Biological Chemistry
Volume266
Issue number11
StatePublished - 1991

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Phosphorylation
Tyrosine
Intercellular Signaling Peptides and Proteins
Phosphotyrosine
NIH 3T3 Cells
Macrophage Colony-Stimulating Factor
Proteins
Macrophages
Granulocyte-Macrophage Colony-Stimulating Factor
Antibodies
Colony-Stimulating Factor Receptors
Cells
Protein Tyrosine Phosphatases
Peptide Mapping
Vanadates
Phosphoprotein Phosphatases
Platelet-Derived Growth Factor
Fibroblasts
Serine
Anti-Idiotypic Antibodies

ASJC Scopus subject areas

  • Biochemistry

Cite this

Tyrosine phosphorylation of a common 57-kDa protein in growth factor-stimulated and -transformed cells. / Li, Wei; Yeung, Y. G.; Stanley, E. Richard.

In: Journal of Biological Chemistry, Vol. 266, No. 11, 1991, p. 6808-6814.

Research output: Contribution to journalArticle

Li, Wei ; Yeung, Y. G. ; Stanley, E. Richard. / Tyrosine phosphorylation of a common 57-kDa protein in growth factor-stimulated and -transformed cells. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 11. pp. 6808-6814.
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