Type-specific and cross-reactive epitopes in human papillomavirus type 16 capsid proteins

Barbara K. Beiss, Edgar Heimer, Arthur Felix, Robert D. Burk, Diane B. Ritter, Robert G. Mallon, Anna Skadish

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Genital human papillomavirus (HPV) 16 infection is frequently associated with cancer of the uterine cervix, as well as with precancerous lesions. In order to generate serologic reagents which might be useful in the diagnosis of HPV 16 infection, rabbit polyclonal and mouse monoclonal antisera were raised to car{ballot box}y terminal peptides from the HPV 16 L1 and L2 open reading frames (ORFs). Anti-L1 and -L2 peptide sera recognized HPV 16 L1 and L2 fusion proteins in Western blots and by immunoprecipitation. In Western blot analysis of L1 proteins from different HPV types, antisera to the L1 peptide reacted only with HPV 16, thus identifying an HPV 16 type-specific linear epitope. Anti-L2 peptide sera reacted with L2 fusion proteins from HPVs 6 and 16, but not from BPV, thus identifying a partially cross-reactive epitope in the HPV 16 L2. Computer analysis of car{ballot box}y terminal amino acid sequences of the L1 and L2 ORFs of multiple HPV types supported the Western blot findings. Despite the HPV 16 type specificity found in Western blots, anti-L1 peptide sera identified nuclear antigen by immunocytochemistry in cervical biopsies infected with HPV 16, as well as other genital HPV types. Anti-L2 peptide sera failed to recognize antigen in infected tissue.

Original languageEnglish (US)
Pages (from-to)460-464
Number of pages5
JournalVirology
Volume184
Issue number1
DOIs
StatePublished - 1991

Fingerprint

Human papillomavirus 16
Capsid Proteins
Epitopes
Peptides
Western Blotting
Papillomavirus Infections
Serum
Open Reading Frames
Immune Sera
Nuclear Antigens
Immunoprecipitation
Uterine Cervical Neoplasms
Amino Acid Sequence
Immunohistochemistry
Rabbits
Biopsy
Antigens

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Beiss, B. K., Heimer, E., Felix, A., Burk, R. D., Ritter, D. B., Mallon, R. G., & Skadish, A. (1991). Type-specific and cross-reactive epitopes in human papillomavirus type 16 capsid proteins. Virology, 184(1), 460-464. https://doi.org/10.1016/0042-6822(91)90870-H

Type-specific and cross-reactive epitopes in human papillomavirus type 16 capsid proteins. / Beiss, Barbara K.; Heimer, Edgar; Felix, Arthur; Burk, Robert D.; Ritter, Diane B.; Mallon, Robert G.; Skadish, Anna.

In: Virology, Vol. 184, No. 1, 1991, p. 460-464.

Research output: Contribution to journalArticle

Beiss, BK, Heimer, E, Felix, A, Burk, RD, Ritter, DB, Mallon, RG & Skadish, A 1991, 'Type-specific and cross-reactive epitopes in human papillomavirus type 16 capsid proteins', Virology, vol. 184, no. 1, pp. 460-464. https://doi.org/10.1016/0042-6822(91)90870-H
Beiss, Barbara K. ; Heimer, Edgar ; Felix, Arthur ; Burk, Robert D. ; Ritter, Diane B. ; Mallon, Robert G. ; Skadish, Anna. / Type-specific and cross-reactive epitopes in human papillomavirus type 16 capsid proteins. In: Virology. 1991 ; Vol. 184, No. 1. pp. 460-464.
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