Two examples of an inseparable antibody that reacts equally well with D(W)+ and Rh32+ red blood cells

Marion E. Reid, Laima Sausais, Charles G. Zaroulis, Kala Mohandas, Gail Coghlan, Christine Lomas-Francis

Research output: Contribution to journalArticle

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Abstract

Background and Objectives: Anti-D(W) is a rare specificity that detects an antigen on D(Va) red blood cells (RBCs). Some anti-D(W) contain an inseparable component that cross-reacts weakly with RBCs expressing the low-incidence Rh antigen Rh32. RH32 is expressed by RBCs with either the R@?(N) or the DBT phenotype. Case Report: We describe here an antibody found in the serum of 2 patients that reacts equally well with RBCs possessing either D(Va), R@?(N), or DBT phenotypes. The reactivity for D(W) and Rh32 antigens could not be separated by adsorption onto and elution from D(W)+Rh32- or from D(W)-Rh32+ RBCs. Conclusions: We suggest that amino acids encoded by nucleotides at the junction of exon 4 of RHD to exon 5 of RHCE may induce a conformation that is recognized by these equally reactive inseparable antibodies. Until such time that the epitope recognized by these antibodies is defined, we recommend use of the descriptive name anti-D(W)/Rh32.

Original languageEnglish (US)
Pages (from-to)230-233
Number of pages4
JournalVox Sanguinis
Volume75
Issue number3
StatePublished - 1998
Externally publishedYes

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Erythrocytes
Antibodies
Antigens
Exons
Phenotype
Adsorption
Names
Epitopes
Nucleotides
Amino Acids
Incidence
Serum
RHO(D) antibody

ASJC Scopus subject areas

  • Hematology

Cite this

Reid, M. E., Sausais, L., Zaroulis, C. G., Mohandas, K., Coghlan, G., & Lomas-Francis, C. (1998). Two examples of an inseparable antibody that reacts equally well with D(W)+ and Rh32+ red blood cells. Vox Sanguinis, 75(3), 230-233.

Two examples of an inseparable antibody that reacts equally well with D(W)+ and Rh32+ red blood cells. / Reid, Marion E.; Sausais, Laima; Zaroulis, Charles G.; Mohandas, Kala; Coghlan, Gail; Lomas-Francis, Christine.

In: Vox Sanguinis, Vol. 75, No. 3, 1998, p. 230-233.

Research output: Contribution to journalArticle

Reid, ME, Sausais, L, Zaroulis, CG, Mohandas, K, Coghlan, G & Lomas-Francis, C 1998, 'Two examples of an inseparable antibody that reacts equally well with D(W)+ and Rh32+ red blood cells', Vox Sanguinis, vol. 75, no. 3, pp. 230-233.
Reid ME, Sausais L, Zaroulis CG, Mohandas K, Coghlan G, Lomas-Francis C. Two examples of an inseparable antibody that reacts equally well with D(W)+ and Rh32+ red blood cells. Vox Sanguinis. 1998;75(3):230-233.
Reid, Marion E. ; Sausais, Laima ; Zaroulis, Charles G. ; Mohandas, Kala ; Coghlan, Gail ; Lomas-Francis, Christine. / Two examples of an inseparable antibody that reacts equally well with D(W)+ and Rh32+ red blood cells. In: Vox Sanguinis. 1998 ; Vol. 75, No. 3. pp. 230-233.
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