Trypanosoma cruzi infection results in the reduced expression of caveolin-3 in the heart

Daniel Adesse, Michael P. Lisanti, David C. Spray, Fabiana S. Machado, Maria De Nazareth Meirelles, Herbert B. Tanowitz, Luciana Ribeiro Garzoni

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Caveolae are motile, membrane-bound compartments that contain a number of molecules that participate in cell signaling. Caveolins are protein markers of caveolae and function in a variety of biological processes. Caveolin-3 (Cav-3) is expressed in muscle cells and Cav-3 null mice display a cardiomyopathic phenotype. Ultrastructural cytochemistry, confocal microscopy and immunoblotting revealed a reduction in Cav-3 expression and an activation of eRK (extracellularsignal-regulated kinase) 48 hours after Trypanosoma cruzi infection of cultured cardiac myocytes. CD-1 mice infected with the Brazil strain of T. cruzi displayed reduced expression of Cav-3 and activation of eRK 66 days post infection (dpi). By 180 dpi there was a normalization of these values. these data suggest that the reduction in Cav-3 expression and the activation of ERK during the early phase of infection may contribute to the pathogenesis of chagasic cardiomyopathy.

Original languageEnglish (US)
Pages (from-to)1639-1646
Number of pages8
JournalCell Cycle
Volume9
Issue number8
StatePublished - Apr 15 2010

Fingerprint

Caveolin 3
Trypanosoma cruzi
Infection
Caveolae
Phosphotransferases
Caveolins
Biological Phenomena
Histocytochemistry
Cardiomyopathies
Immunoblotting
Cardiac Myocytes
Confocal Microscopy
Muscle Cells
Brazil
Phenotype
Membranes

Keywords

  • Cardiac myocytes
  • Caveolae
  • Caveolin-3
  • ERK
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Cite this

Adesse, D., Lisanti, M. P., Spray, D. C., Machado, F. S., Meirelles, M. D. N., Tanowitz, H. B., & Garzoni, L. R. (2010). Trypanosoma cruzi infection results in the reduced expression of caveolin-3 in the heart. Cell Cycle, 9(8), 1639-1646.

Trypanosoma cruzi infection results in the reduced expression of caveolin-3 in the heart. / Adesse, Daniel; Lisanti, Michael P.; Spray, David C.; Machado, Fabiana S.; Meirelles, Maria De Nazareth; Tanowitz, Herbert B.; Garzoni, Luciana Ribeiro.

In: Cell Cycle, Vol. 9, No. 8, 15.04.2010, p. 1639-1646.

Research output: Contribution to journalArticle

Adesse, D, Lisanti, MP, Spray, DC, Machado, FS, Meirelles, MDN, Tanowitz, HB & Garzoni, LR 2010, 'Trypanosoma cruzi infection results in the reduced expression of caveolin-3 in the heart', Cell Cycle, vol. 9, no. 8, pp. 1639-1646.
Adesse D, Lisanti MP, Spray DC, Machado FS, Meirelles MDN, Tanowitz HB et al. Trypanosoma cruzi infection results in the reduced expression of caveolin-3 in the heart. Cell Cycle. 2010 Apr 15;9(8):1639-1646.
Adesse, Daniel ; Lisanti, Michael P. ; Spray, David C. ; Machado, Fabiana S. ; Meirelles, Maria De Nazareth ; Tanowitz, Herbert B. ; Garzoni, Luciana Ribeiro. / Trypanosoma cruzi infection results in the reduced expression of caveolin-3 in the heart. In: Cell Cycle. 2010 ; Vol. 9, No. 8. pp. 1639-1646.
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