Trypanosoma cruzi induces endothelin release from endothelial cells

M. Wittner, G. J. Christ, Huan Huang, Louis M. Weiss, Victor Bernard Hatcher, S. A. Morris, G. A. Orr, Joan W. Berman, G. A. Zeballos, S. A. Douglas, H. B. Tanowitz

Research output: Contribution to journalArticle

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Abstract

The potential role of endothelin-1 (ET-1) in the pathogenesis of focal microvascular spasm, previously implicated in the etiology of Chagas' cardiomyopathy, was investigated. There was an increase in ET-1 in the supernatants of Trypanosoma cruzi-infected human umbilical vein endothelial cells (HUVEC). Infection of HUVEC and vascular smooth muscle cells had no effect on the synthesis of transforming growth factor-β, which induces ET-1 synthesis. Bioassay studies of isolated rat aortic rings revealed that the increases in ET-1 production were associated with augmented contractile responses, which were significantly attenuated by preincubation with the ET(A) receptor antagonist, BQ-123. When big ET was incubated with the parasite, there was no conversion of the precursor to the active hormone (ET- 1), demonstrating that the parasite did not possess the necessary converting enzyme. These observations suggest the potential importance of ET-1 in the etiology of the microvascular spasm associated with Chagas' disease.

Original languageEnglish (US)
Pages (from-to)493-497
Number of pages5
JournalJournal of Infectious Diseases
Volume171
Issue number2
StatePublished - 1995

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Trypanosoma cruzi
Endothelins
Endothelin-1
Endothelial Cells
Human Umbilical Vein Endothelial Cells
Spasm
Parasites
Chagas Cardiomyopathy
Chagas Disease
Transforming Growth Factors
Vascular Smooth Muscle
Biological Assay
Smooth Muscle Myocytes
Hormones
Enzymes
Infection

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Wittner, M., Christ, G. J., Huang, H., Weiss, L. M., Hatcher, V. B., Morris, S. A., ... Tanowitz, H. B. (1995). Trypanosoma cruzi induces endothelin release from endothelial cells. Journal of Infectious Diseases, 171(2), 493-497.

Trypanosoma cruzi induces endothelin release from endothelial cells. / Wittner, M.; Christ, G. J.; Huang, Huan; Weiss, Louis M.; Hatcher, Victor Bernard; Morris, S. A.; Orr, G. A.; Berman, Joan W.; Zeballos, G. A.; Douglas, S. A.; Tanowitz, H. B.

In: Journal of Infectious Diseases, Vol. 171, No. 2, 1995, p. 493-497.

Research output: Contribution to journalArticle

Wittner, M, Christ, GJ, Huang, H, Weiss, LM, Hatcher, VB, Morris, SA, Orr, GA, Berman, JW, Zeballos, GA, Douglas, SA & Tanowitz, HB 1995, 'Trypanosoma cruzi induces endothelin release from endothelial cells', Journal of Infectious Diseases, vol. 171, no. 2, pp. 493-497.
Wittner, M. ; Christ, G. J. ; Huang, Huan ; Weiss, Louis M. ; Hatcher, Victor Bernard ; Morris, S. A. ; Orr, G. A. ; Berman, Joan W. ; Zeballos, G. A. ; Douglas, S. A. ; Tanowitz, H. B. / Trypanosoma cruzi induces endothelin release from endothelial cells. In: Journal of Infectious Diseases. 1995 ; Vol. 171, No. 2. pp. 493-497.
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