Treatment of experimental acute severe anemia with parenteral iron

Feng Qin, Aryeh Shander, Manoj Mammen, Jennifer W. Chuy, Herbert Dardik

Research output: Contribution to journalArticle

Abstract

INTRUDUCTIONS: Unpredictable significant blood loss is encountered in cases of severe traumas or major surgeries. A quicker recovery of hemoglobin (Hb) would reduce or eliminate the possibility exposing to allogeneic transfusion. The purpose of this study is to evaluate the effects of parenteral iron on experimental acute severe anemia. METHODS: 50 Sprague-Dawley rats, weighing 350g, were used in the study. Blood was removed through the carotid catheter and replaced by 6% hetastarch sodium. The Hb was reduced 50% on first day. The following day, blood depletion continued until Hb were reduced to 30% (4mg/dL) of that of normal rats. Blood pressure and heart rate were maintained within normal range. The anemic rats were randomly divided into two groups: the control group without any treatment and the iron group, in which the high dose (3mg/kg/day) of Iron dextran was given i.v. daily for 10 days postphlebotomy. Hb, hematocrit (Hct), and reticulocyte counts (Ret) as wen as serum iron were tested from baseline to 28 days postphlebotomy. RESULTS: Hb in both groups showed a gradual increase. The Hb in the iron group grew faster than that in the control group between days 6 and 10 (p < 0.05). It took 12 or 14 days (the iron-treated group, the control group, respectably) for Hb s recovery to normal range. Hct were higher in the iron therapy group than those in the control group (p < 0.05) between days 5 and 10. The Ret in the two groups increased after phlebotomy. Ret in the iron therapy group remained higher than those of the control group (p < 0.05) between days 3 and 7. The decrease of circulating iron after phlebotomy was induced. Once replenished with parenteral iron, circulating iron was higher than that of the control group (p < 0.05). CONCLUSIONS: Parenteral iron in large doses can maintain the higher levels of serum iron, increase the Ret, elevate the Hb and Hct faster, and shorten the recovery period after acute severe anemia. It reduces the possibility of allogeneic blood exposure. Also, we speculate that iron has two different roles in increasing Hb concentration: 1) iron is a substrate for heme synthesis, and 2) iron directly modulates the process of erythropoiesis.

Original languageEnglish (US)
JournalCritical Care Medicine
Volume27
Issue number12 SUPPL.
StatePublished - 1999
Externally publishedYes

Fingerprint

Anemia
Iron
Hemoglobins
Control Groups
Hematocrit
Phlebotomy
Group Psychotherapy
Reference Values
Hydroxyethyl Starch Derivatives
Reticulocyte Count
Erythropoiesis
Dextrans
Serum
Heme
Sprague Dawley Rats
Catheters
Heart Rate
Sodium

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Qin, F., Shander, A., Mammen, M., Chuy, J. W., & Dardik, H. (1999). Treatment of experimental acute severe anemia with parenteral iron. Critical Care Medicine, 27(12 SUPPL.).

Treatment of experimental acute severe anemia with parenteral iron. / Qin, Feng; Shander, Aryeh; Mammen, Manoj; Chuy, Jennifer W.; Dardik, Herbert.

In: Critical Care Medicine, Vol. 27, No. 12 SUPPL., 1999.

Research output: Contribution to journalArticle

Qin, F, Shander, A, Mammen, M, Chuy, JW & Dardik, H 1999, 'Treatment of experimental acute severe anemia with parenteral iron', Critical Care Medicine, vol. 27, no. 12 SUPPL..
Qin, Feng ; Shander, Aryeh ; Mammen, Manoj ; Chuy, Jennifer W. ; Dardik, Herbert. / Treatment of experimental acute severe anemia with parenteral iron. In: Critical Care Medicine. 1999 ; Vol. 27, No. 12 SUPPL.
@article{2ca6a0edd9044656bfef9ba027e4a7c9,
title = "Treatment of experimental acute severe anemia with parenteral iron",
abstract = "INTRUDUCTIONS: Unpredictable significant blood loss is encountered in cases of severe traumas or major surgeries. A quicker recovery of hemoglobin (Hb) would reduce or eliminate the possibility exposing to allogeneic transfusion. The purpose of this study is to evaluate the effects of parenteral iron on experimental acute severe anemia. METHODS: 50 Sprague-Dawley rats, weighing 350g, were used in the study. Blood was removed through the carotid catheter and replaced by 6{\%} hetastarch sodium. The Hb was reduced 50{\%} on first day. The following day, blood depletion continued until Hb were reduced to 30{\%} (4mg/dL) of that of normal rats. Blood pressure and heart rate were maintained within normal range. The anemic rats were randomly divided into two groups: the control group without any treatment and the iron group, in which the high dose (3mg/kg/day) of Iron dextran was given i.v. daily for 10 days postphlebotomy. Hb, hematocrit (Hct), and reticulocyte counts (Ret) as wen as serum iron were tested from baseline to 28 days postphlebotomy. RESULTS: Hb in both groups showed a gradual increase. The Hb in the iron group grew faster than that in the control group between days 6 and 10 (p < 0.05). It took 12 or 14 days (the iron-treated group, the control group, respectably) for Hb s recovery to normal range. Hct were higher in the iron therapy group than those in the control group (p < 0.05) between days 5 and 10. The Ret in the two groups increased after phlebotomy. Ret in the iron therapy group remained higher than those of the control group (p < 0.05) between days 3 and 7. The decrease of circulating iron after phlebotomy was induced. Once replenished with parenteral iron, circulating iron was higher than that of the control group (p < 0.05). CONCLUSIONS: Parenteral iron in large doses can maintain the higher levels of serum iron, increase the Ret, elevate the Hb and Hct faster, and shorten the recovery period after acute severe anemia. It reduces the possibility of allogeneic blood exposure. Also, we speculate that iron has two different roles in increasing Hb concentration: 1) iron is a substrate for heme synthesis, and 2) iron directly modulates the process of erythropoiesis.",
author = "Feng Qin and Aryeh Shander and Manoj Mammen and Chuy, {Jennifer W.} and Herbert Dardik",
year = "1999",
language = "English (US)",
volume = "27",
journal = "Critical Care Medicine",
issn = "0090-3493",
publisher = "Lippincott Williams and Wilkins",
number = "12 SUPPL.",

}

TY - JOUR

T1 - Treatment of experimental acute severe anemia with parenteral iron

AU - Qin, Feng

AU - Shander, Aryeh

AU - Mammen, Manoj

AU - Chuy, Jennifer W.

AU - Dardik, Herbert

PY - 1999

Y1 - 1999

N2 - INTRUDUCTIONS: Unpredictable significant blood loss is encountered in cases of severe traumas or major surgeries. A quicker recovery of hemoglobin (Hb) would reduce or eliminate the possibility exposing to allogeneic transfusion. The purpose of this study is to evaluate the effects of parenteral iron on experimental acute severe anemia. METHODS: 50 Sprague-Dawley rats, weighing 350g, were used in the study. Blood was removed through the carotid catheter and replaced by 6% hetastarch sodium. The Hb was reduced 50% on first day. The following day, blood depletion continued until Hb were reduced to 30% (4mg/dL) of that of normal rats. Blood pressure and heart rate were maintained within normal range. The anemic rats were randomly divided into two groups: the control group without any treatment and the iron group, in which the high dose (3mg/kg/day) of Iron dextran was given i.v. daily for 10 days postphlebotomy. Hb, hematocrit (Hct), and reticulocyte counts (Ret) as wen as serum iron were tested from baseline to 28 days postphlebotomy. RESULTS: Hb in both groups showed a gradual increase. The Hb in the iron group grew faster than that in the control group between days 6 and 10 (p < 0.05). It took 12 or 14 days (the iron-treated group, the control group, respectably) for Hb s recovery to normal range. Hct were higher in the iron therapy group than those in the control group (p < 0.05) between days 5 and 10. The Ret in the two groups increased after phlebotomy. Ret in the iron therapy group remained higher than those of the control group (p < 0.05) between days 3 and 7. The decrease of circulating iron after phlebotomy was induced. Once replenished with parenteral iron, circulating iron was higher than that of the control group (p < 0.05). CONCLUSIONS: Parenteral iron in large doses can maintain the higher levels of serum iron, increase the Ret, elevate the Hb and Hct faster, and shorten the recovery period after acute severe anemia. It reduces the possibility of allogeneic blood exposure. Also, we speculate that iron has two different roles in increasing Hb concentration: 1) iron is a substrate for heme synthesis, and 2) iron directly modulates the process of erythropoiesis.

AB - INTRUDUCTIONS: Unpredictable significant blood loss is encountered in cases of severe traumas or major surgeries. A quicker recovery of hemoglobin (Hb) would reduce or eliminate the possibility exposing to allogeneic transfusion. The purpose of this study is to evaluate the effects of parenteral iron on experimental acute severe anemia. METHODS: 50 Sprague-Dawley rats, weighing 350g, were used in the study. Blood was removed through the carotid catheter and replaced by 6% hetastarch sodium. The Hb was reduced 50% on first day. The following day, blood depletion continued until Hb were reduced to 30% (4mg/dL) of that of normal rats. Blood pressure and heart rate were maintained within normal range. The anemic rats were randomly divided into two groups: the control group without any treatment and the iron group, in which the high dose (3mg/kg/day) of Iron dextran was given i.v. daily for 10 days postphlebotomy. Hb, hematocrit (Hct), and reticulocyte counts (Ret) as wen as serum iron were tested from baseline to 28 days postphlebotomy. RESULTS: Hb in both groups showed a gradual increase. The Hb in the iron group grew faster than that in the control group between days 6 and 10 (p < 0.05). It took 12 or 14 days (the iron-treated group, the control group, respectably) for Hb s recovery to normal range. Hct were higher in the iron therapy group than those in the control group (p < 0.05) between days 5 and 10. The Ret in the two groups increased after phlebotomy. Ret in the iron therapy group remained higher than those of the control group (p < 0.05) between days 3 and 7. The decrease of circulating iron after phlebotomy was induced. Once replenished with parenteral iron, circulating iron was higher than that of the control group (p < 0.05). CONCLUSIONS: Parenteral iron in large doses can maintain the higher levels of serum iron, increase the Ret, elevate the Hb and Hct faster, and shorten the recovery period after acute severe anemia. It reduces the possibility of allogeneic blood exposure. Also, we speculate that iron has two different roles in increasing Hb concentration: 1) iron is a substrate for heme synthesis, and 2) iron directly modulates the process of erythropoiesis.

UR - http://www.scopus.com/inward/record.url?scp=33750645695&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750645695&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33750645695

VL - 27

JO - Critical Care Medicine

JF - Critical Care Medicine

SN - 0090-3493

IS - 12 SUPPL.

ER -