TY - JOUR
T1 - Treatment of Caenorhabditis elegans with Small Selenium Species Enhances Antioxidant Defense Systems
AU - Rohn, Isabelle
AU - Raschke, Stefanie
AU - Aschner, Michael
AU - Tuck, Simon
AU - Kuehnelt, Doris
AU - Kipp, Anna
AU - Schwerdtle, Tanja
AU - Bornhorst, Julia
N1 - Publisher Copyright:
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2019/5
Y1 - 2019/5
N2 - Scope: Small selenium (Se) species play a key role in Se metabolism and act as dietary sources of the essential trace element. However, they are redox-active and trigger pro- and antioxidant responses. As health outcomes are strongly species-dependent, species-specific characteristics of Se compounds are tested in vivo. Methods and results: In the model organism Caenorhabditis elegans (C. elegans), immediate and sustained effects of selenite, selenomethionine (SeMet), and Se-methylselenocysteine (MeSeCys) are studied regarding their bioavailability, incorporation into proteins, as well as modulation of the cellular redox status. While all tested Se compounds are bioavailable, only SeMet persistently accumulates and is non-specifically incorporated into proteins. However, the protection toward chemically-induced formation of reactive species is independent of the applied Se compound. Increased thioredoxin reductase (TXNRD) activity and changes in mRNA expression levels of antioxidant proteins indicate the activation of cellular defense mechanisms. However, in txnrd-1 deletion mutants, no protective effects of the Se species are observed anymore, which is also reflected by differential gene expression data. Conclusion: Se species protect against chemically-induced reactive species formation. The identified immediate and sustained systemic effects of Se species give rise to speculations on possible benefits facing subsequent periods of inadequate Se intake.
AB - Scope: Small selenium (Se) species play a key role in Se metabolism and act as dietary sources of the essential trace element. However, they are redox-active and trigger pro- and antioxidant responses. As health outcomes are strongly species-dependent, species-specific characteristics of Se compounds are tested in vivo. Methods and results: In the model organism Caenorhabditis elegans (C. elegans), immediate and sustained effects of selenite, selenomethionine (SeMet), and Se-methylselenocysteine (MeSeCys) are studied regarding their bioavailability, incorporation into proteins, as well as modulation of the cellular redox status. While all tested Se compounds are bioavailable, only SeMet persistently accumulates and is non-specifically incorporated into proteins. However, the protection toward chemically-induced formation of reactive species is independent of the applied Se compound. Increased thioredoxin reductase (TXNRD) activity and changes in mRNA expression levels of antioxidant proteins indicate the activation of cellular defense mechanisms. However, in txnrd-1 deletion mutants, no protective effects of the Se species are observed anymore, which is also reflected by differential gene expression data. Conclusion: Se species protect against chemically-induced reactive species formation. The identified immediate and sustained systemic effects of Se species give rise to speculations on possible benefits facing subsequent periods of inadequate Se intake.
KW - antioxidant defense systems
KW - caenorhabditis elegans
KW - oxidative stress
KW - selenium
KW - selenoproteins
UR - http://www.scopus.com/inward/record.url?scp=85062975493&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85062975493&partnerID=8YFLogxK
U2 - 10.1002/mnfr.201801304
DO - 10.1002/mnfr.201801304
M3 - Article
C2 - 30815971
AN - SCOPUS:85062975493
SN - 1613-4125
VL - 63
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
IS - 9
M1 - 1801304
ER -