Treatment of Caenorhabditis elegans with Small Selenium Species Enhances Antioxidant Defense Systems

Isabelle Rohn, Stefanie Raschke, Michael Aschner, Simon Tuck, Doris Kuehnelt, Anna Kipp, Tanja Schwerdtle, Julia Bornhorst

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Scope: Small selenium (Se) species play a key role in Se metabolism and act as dietary sources of the essential trace element. However, they are redox-active and trigger pro- and antioxidant responses. As health outcomes are strongly species-dependent, species-specific characteristics of Se compounds are tested in vivo. Methods and results: In the model organism Caenorhabditis elegans (C. elegans), immediate and sustained effects of selenite, selenomethionine (SeMet), and Se-methylselenocysteine (MeSeCys) are studied regarding their bioavailability, incorporation into proteins, as well as modulation of the cellular redox status. While all tested Se compounds are bioavailable, only SeMet persistently accumulates and is non-specifically incorporated into proteins. However, the protection toward chemically-induced formation of reactive species is independent of the applied Se compound. Increased thioredoxin reductase (TXNRD) activity and changes in mRNA expression levels of antioxidant proteins indicate the activation of cellular defense mechanisms. However, in txnrd-1 deletion mutants, no protective effects of the Se species are observed anymore, which is also reflected by differential gene expression data. Conclusion: Se species protect against chemically-induced reactive species formation. The identified immediate and sustained systemic effects of Se species give rise to speculations on possible benefits facing subsequent periods of inadequate Se intake.

Original languageEnglish (US)
Article number1801304
JournalMolecular Nutrition and Food Research
DOIs
StatePublished - Jan 1 2019

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Caenorhabditis elegans
Selenium
selenium
Antioxidants
antioxidant activity
Selenium Compounds
Selenomethionine
selenomethionine
Oxidation-Reduction
Thioredoxin-Disulfide Reductase
Selenious Acid
Proteins
Trace Elements
Biological Availability
antioxidants
selenites
proteins
defense mechanisms
Gene Expression
protective effect

Keywords

  • antioxidant defense systems
  • caenorhabditis elegans
  • oxidative stress
  • selenium
  • selenoproteins

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

Cite this

Treatment of Caenorhabditis elegans with Small Selenium Species Enhances Antioxidant Defense Systems. / Rohn, Isabelle; Raschke, Stefanie; Aschner, Michael; Tuck, Simon; Kuehnelt, Doris; Kipp, Anna; Schwerdtle, Tanja; Bornhorst, Julia.

In: Molecular Nutrition and Food Research, 01.01.2019.

Research output: Contribution to journalArticle

Rohn, Isabelle ; Raschke, Stefanie ; Aschner, Michael ; Tuck, Simon ; Kuehnelt, Doris ; Kipp, Anna ; Schwerdtle, Tanja ; Bornhorst, Julia. / Treatment of Caenorhabditis elegans with Small Selenium Species Enhances Antioxidant Defense Systems. In: Molecular Nutrition and Food Research. 2019.
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abstract = "Scope: Small selenium (Se) species play a key role in Se metabolism and act as dietary sources of the essential trace element. However, they are redox-active and trigger pro- and antioxidant responses. As health outcomes are strongly species-dependent, species-specific characteristics of Se compounds are tested in vivo. Methods and results: In the model organism Caenorhabditis elegans (C. elegans), immediate and sustained effects of selenite, selenomethionine (SeMet), and Se-methylselenocysteine (MeSeCys) are studied regarding their bioavailability, incorporation into proteins, as well as modulation of the cellular redox status. While all tested Se compounds are bioavailable, only SeMet persistently accumulates and is non-specifically incorporated into proteins. However, the protection toward chemically-induced formation of reactive species is independent of the applied Se compound. Increased thioredoxin reductase (TXNRD) activity and changes in mRNA expression levels of antioxidant proteins indicate the activation of cellular defense mechanisms. However, in txnrd-1 deletion mutants, no protective effects of the Se species are observed anymore, which is also reflected by differential gene expression data. Conclusion: Se species protect against chemically-induced reactive species formation. The identified immediate and sustained systemic effects of Se species give rise to speculations on possible benefits facing subsequent periods of inadequate Se intake.",
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