Transparent Adult Zebrafish as a Tool for In Vivo Transplantation Analysis

Richard Mark White, Anna Sessa, Christopher Burke, Teresa Bowman, Jocelyn LeBlanc, Craig Ceol, Caitlin Bourque, Michael Dovey, Wolfram Goessling, Caroline Erter Burns, Leonard I. Zon

Research output: Contribution to journalArticle

607 Scopus citations

Abstract

The zebrafish is a useful model for understanding normal and cancer stem cells, but analysis has been limited to embryogenesis due to the opacity of the adult fish. To address this, we have created a transparent adult zebrafish in which we transplanted either hematopoietic stem/progenitor cells or tumor cells. In a hematopoiesis radiation recovery assay, transplantation of GFP-labeled marrow cells allowed for striking in vivo visual assessment of engraftment from 2 hr-5 weeks posttransplant. Using FACS analysis, both transparent and wild-type fish had equal engraftment, but this could only be visualized in the transparent recipient. In a tumor engraftment model, transplantation of RAS-melanoma cells allowed for visualization of tumor engraftment, proliferation, and distant metastases in as little as 5 days, which is not seen in wild-type recipients until 3 to 4 weeks. This transparent adult zebrafish serves as the ideal combination of both sensitivity and resolution for in vivo stem cell analyses.

Original languageEnglish (US)
Pages (from-to)183-189
Number of pages7
JournalCell Stem Cell
Volume2
Issue number2
DOIs
StatePublished - Feb 7 2008
Externally publishedYes

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Keywords

  • DEVBIO
  • STEMCELL

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Cell Biology

Cite this

White, R. M., Sessa, A., Burke, C., Bowman, T., LeBlanc, J., Ceol, C., Bourque, C., Dovey, M., Goessling, W., Burns, C. E., & Zon, L. I. (2008). Transparent Adult Zebrafish as a Tool for In Vivo Transplantation Analysis. Cell Stem Cell, 2(2), 183-189. https://doi.org/10.1016/j.stem.2007.11.002