Translated Alu sequence determines nuclear localization of a novel catalytic subunit of casein kinase 2

Philip Hilgard, Tianmin Huang, Allan W. Wolkoff, Richard J. Stockert

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Casein kinase 2 (CK2) is a tetrameric enzyme constitutively expressed in all eukaryotic tissues. The two known isoforms of the catalytic subunit, CK2α and CK2α′, have been reported to have distinct tissue-dependent subcellular distributions. We recently described a third isoform of the catalytic subunit, designated CK2α″, which is highly expressed in liver. Immunoblot analysis of HuH-7 human hepatoma cell fractions as well as immunofluorescent microscopy revealed that CK2α″ was exclusively localized to the nucleus and preferentially associated with the nuclear matrix. CK2α and CK2α′ were found in nuclear, membrane, and cytosolic compartments. Deletion of the carboxy-terminal 32 amino acids from the CK2α″ sequence resulted in release of the truncated green fluorescent protein fusion protein from the nuclear matrix and redistribution to both the nucleus and the cytoplasm. Demonstration that the carboxy terminus is necessary but not sufficient for nuclear retention indicates that the underlying mechanism of CK2α″ nuclear localization is dependent on the secondary structure of the holoenzyme directed by the carboxy-terminal sequence.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume283
Issue number2 52-2
StatePublished - 2002

Fingerprint

Casein Kinase II
Catalytic Domain
Protein Isoforms
Nuclear Matrix-Associated Proteins
Tissue
Nuclear Matrix
Holoenzymes
Nuclear Envelope
Green Fluorescent Proteins
Liver
Microscopy
Hepatocellular Carcinoma
Microscopic examination
Cytoplasm
Demonstrations
Fusion reactions
Membranes
Amino Acids

Keywords

  • Casein kinase 2-green fluorescent protein fusion protein
  • Human hepatoma cell line HuH-7
  • Nuclear matrix association

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Translated Alu sequence determines nuclear localization of a novel catalytic subunit of casein kinase 2. / Hilgard, Philip; Huang, Tianmin; Wolkoff, Allan W.; Stockert, Richard J.

In: American Journal of Physiology - Cell Physiology, Vol. 283, No. 2 52-2, 2002.

Research output: Contribution to journalArticle

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