TY - JOUR
T1 - Translated Alu sequence determines nuclear localization of a novel catalytic subunit of casein kinase 2
AU - Hilgard, Philip
AU - Huang, Tianmin
AU - Wolkoff, Allan W.
AU - Stockert, Richard J.
PY - 2002
Y1 - 2002
N2 - Casein kinase 2 (CK2) is a tetrameric enzyme constitutively expressed in all eukaryotic tissues. The two known isoforms of the catalytic subunit, CK2α and CK2α′, have been reported to have distinct tissue-dependent subcellular distributions. We recently described a third isoform of the catalytic subunit, designated CK2α″, which is highly expressed in liver. Immunoblot analysis of HuH-7 human hepatoma cell fractions as well as immunofluorescent microscopy revealed that CK2α″ was exclusively localized to the nucleus and preferentially associated with the nuclear matrix. CK2α and CK2α′ were found in nuclear, membrane, and cytosolic compartments. Deletion of the carboxy-terminal 32 amino acids from the CK2α″ sequence resulted in release of the truncated green fluorescent protein fusion protein from the nuclear matrix and redistribution to both the nucleus and the cytoplasm. Demonstration that the carboxy terminus is necessary but not sufficient for nuclear retention indicates that the underlying mechanism of CK2α″ nuclear localization is dependent on the secondary structure of the holoenzyme directed by the carboxy-terminal sequence.
AB - Casein kinase 2 (CK2) is a tetrameric enzyme constitutively expressed in all eukaryotic tissues. The two known isoforms of the catalytic subunit, CK2α and CK2α′, have been reported to have distinct tissue-dependent subcellular distributions. We recently described a third isoform of the catalytic subunit, designated CK2α″, which is highly expressed in liver. Immunoblot analysis of HuH-7 human hepatoma cell fractions as well as immunofluorescent microscopy revealed that CK2α″ was exclusively localized to the nucleus and preferentially associated with the nuclear matrix. CK2α and CK2α′ were found in nuclear, membrane, and cytosolic compartments. Deletion of the carboxy-terminal 32 amino acids from the CK2α″ sequence resulted in release of the truncated green fluorescent protein fusion protein from the nuclear matrix and redistribution to both the nucleus and the cytoplasm. Demonstration that the carboxy terminus is necessary but not sufficient for nuclear retention indicates that the underlying mechanism of CK2α″ nuclear localization is dependent on the secondary structure of the holoenzyme directed by the carboxy-terminal sequence.
KW - Casein kinase 2-green fluorescent protein fusion protein
KW - Human hepatoma cell line HuH-7
KW - Nuclear matrix association
UR - http://www.scopus.com/inward/record.url?scp=0036077214&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036077214&partnerID=8YFLogxK
U2 - 10.1152/ajpcell.00070.2002
DO - 10.1152/ajpcell.00070.2002
M3 - Article
C2 - 12107057
AN - SCOPUS:0036077214
SN - 0363-6143
VL - 283
SP - C472-C483
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
IS - 2 52-2
ER -