Transfer of blood urea nitrogen to cecal microbial nitrogen is increased by fructo-oligosaccharide feeding in guinea pigs

Kiyonori Kawasaki, Xiao Min, Alus Michael Xiaoli, Ena Hasegawa, Ei Sakaguchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The present study was conducted to determine the mechanism by which nitrogen (N) availability is improved by fructo-oligosaccharide (FOS) in guinea pigs. Adult male guinea pigs were fed a commercial pellet diet (50g/day) with either 5% glucose or 5% FOS for 7 days in individual metabolism cages. After 7 days of feeding the diet, 15N-urea was administered intravenously 1h before slaughter under anesthesia. The amount and concentration of total, protein, bacterial, ammonia and urea N and the 15N atom % excess were measured in blood, liver, gut contents and urine. The 15N atom % excess of total and protein N, and the amount of total, protein and bacteria N and 15N in the cecum were significantly increased by the consumption of FOS. Furthermore, the concentration and amount of short-chain fatty acids were significantly increased by the consumption of FOS. In contrast, the amount of urinary 15N was significantly decreased by the consumption of FOS. These results suggest that consumption of FOS increases transfer of blood urea N into the large intestine for bacterial N synthesis, which is subsequently re-absorbed by cecotrophy, and contributes to the increase of N utilization in guinea pigs.

Original languageEnglish (US)
Pages (from-to)77-82
Number of pages6
JournalAnimal Science Journal
Volume86
Issue number1
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

fructooligosaccharides
Blood Urea Nitrogen
urea nitrogen
Oligosaccharides
guinea pigs
Guinea Pigs
Nitrogen
blood
nitrogen
Urea
urea
Diet
bacterial proteins
Bacterial Proteins
Cecum
Volatile Fatty Acids
Large Intestine
short chain fatty acids
large intestine
Ammonia

Keywords

  • N-urea
  • Cecum
  • Fructo-oligosaccharide
  • Guinea pig
  • Nitrogen utilization

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Transfer of blood urea nitrogen to cecal microbial nitrogen is increased by fructo-oligosaccharide feeding in guinea pigs. / Kawasaki, Kiyonori; Min, Xiao; Xiaoli, Alus Michael; Hasegawa, Ena; Sakaguchi, Ei.

In: Animal Science Journal, Vol. 86, No. 1, 01.01.2015, p. 77-82.

Research output: Contribution to journalArticle

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