Transfection of a human gene that corrects the Lec1 glycosylation defect: Evidence for transfer of the structural gene for N-acetylglucosaminyltransferase I

R. Kumar, P. Stanley

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Abstract

Chinese hamster ovary (CHO) glycosylation mutants provide an approach to cloning mammalian glycosyltransferases by transfection and gene rescue. In this paper, complementation of the lec1 CHO mutation by human DNA is described. Lec1 transfectants expressed human N-acetylglucosaminyltransferase I (GlcNAc-TI) activity and possessed common human DNA fragments. Cloning of GlcNAc-TI should therefore be possible.

Original languageEnglish (US)
Pages (from-to)5713-5717
Number of pages5
JournalMolecular and cellular biology
Volume9
Issue number12
DOIs
StatePublished - Jan 1 1989

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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