TY - JOUR
T1 - Transfection of a human gene that corrects the Lec1 glycosylation defect
T2 - Evidence for transfer of the structural gene for N-acetylglucosaminyltransferase I
AU - Kumar, R.
AU - Stanley, P.
PY - 1989
Y1 - 1989
N2 - Chinese hamster ovary (CHO) glycosylation mutants provide an approach to cloning mammalian glycosyltransferases by transfection and gene rescue. In this paper, complementation of the lec1 CHO mutation by human DNA is described. Lec1 transfectants expressed human N-acetylglucosaminyltransferase I (GlcNAc-TI) activity and possessed common human DNA fragments. Cloning of GlcNAc-TI should therefore be possible.
AB - Chinese hamster ovary (CHO) glycosylation mutants provide an approach to cloning mammalian glycosyltransferases by transfection and gene rescue. In this paper, complementation of the lec1 CHO mutation by human DNA is described. Lec1 transfectants expressed human N-acetylglucosaminyltransferase I (GlcNAc-TI) activity and possessed common human DNA fragments. Cloning of GlcNAc-TI should therefore be possible.
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U2 - 10.1128/MCB.9.12.5713
DO - 10.1128/MCB.9.12.5713
M3 - Article
C2 - 2531285
AN - SCOPUS:0024408670
SN - 0270-7306
VL - 9
SP - 5713
EP - 5717
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 12
ER -