Abstract
Classical insulin and insulin-like growth factor I (IGF-I) receptors exist as well defined α2β2 heterotetrameric complexes that are assembled from two identical αβ heterodimeric half-receptor precursors. Recent evidence suggests that insulin and IGF-I half-receptors can heterologously assemble to form α2β2 insulin/IGF-I hybrid receptor complexes in vivo and in vitro. We have utilized hybrid receptor complexes to examine ligand-stimulated transmembrane signaling of wild-type insulin (αβINS.WT) or IGF-I (αβIGF.WT) half-receptors assembled with a kinase-defective insulin half-receptor mutant (αβINS.A/K). In vitro assembly of either (αβ)IGF.WT/(αβ)INS.A/K or (αβ)INS.WT/(αβ)INS.A/K hybrid receptors resulted in decreased substrate protein kinase activity. The degree of protein kinase inactivation directly correlated with the amount of immunologically cross-reactive hybrid receptors formed. In contrast to substrate kinase activity, insulin-stimulated autophosphorylation of the (αβ)INS.WT/(αβ)INS.A/K hybrid receptor complex was completely unaffected in comparison to the wild-type (αβ)INS.WT/(αβ)INS.WT receptor. To assess a molecular basis for this difference, autophosphorylation of a hybrid receptor composed of a truncated β-subunit insulin half-receptor with the kinase-defective half-receptor, (αβ)INS.ΔCT/ (αβ)INS.A/K, demonstrated the exclusive autophosphorylation of the (αβ)INS.A/K half-receptor β subunit. These results demonstrate that ligand-dependent substrate phosphorylation by insulin and IGF-I holoreceptors requires interactions between two functional β subunits within the α2β2 heterotetrameric complex and occurs through an intramolecular trans-phosphorylation reaction.
Original language | English (US) |
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Pages (from-to) | 214-218 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 88 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 1991 |
Externally published | Yes |
Keywords
- Autophosphorylation
- Heterologous receptors
- In vitro assembly
- Substrate phosphorylation
ASJC Scopus subject areas
- General