Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis

Ariel Herrera-Vásquez, Loreto Carvallo, Francisca Blanco, Mariola Tobar, Eva Villarroel-Candia, Jesús Vicente-Carbajosa, Paula Salinas, Loreto Holuigue

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Salicylic acid (SA) is a key hormone that mediates gene transcriptional reprogramming in the context of the defense response to stress. GRXC9, coding for a CC-type glutaredoxin from Arabidopsis, is an SA-responsive gene induced early and transiently by an NPR1-independent pathway. Here, we address the mechanism involved in this SA-dependent pathway, using GRXC9 as a model gene. We first established that GRXC9 expression is induced by UVB exposure through this pathway, validating its activation in a physiological stress condition. GRXC9 promoter analyses indicate that SA controls gene transcription through two activating sequence-1 (as-1)-like elements located in its proximal region. TGA2 and TGA3, but not TGA1, are constitutively bound to this promoter region. Accordingly, the transient recruitment of RNA polymerase II to the GRXC9 promoter, as well as the transient accumulation of gene transcripts detected in SA-treated WT plants, was abolished in a knockout mutant for the TGA class II factors. We conclude that constitutive binding of TGA2 is essential for controlling GRXC9 expression, while binding of TGA3 in a lesser extent contributes to this regulation. Finally, overexpression of GRXC9 indicates that the GRXC9 protein negatively controls its own gene expression, forming part of the complex bound to the as-1-containing promoter region. These findings are integrated in a model that explains how SA controls transcription of GRXC9 in the context of the defense response to stress.

Original languageEnglish (US)
Pages (from-to)624-637
Number of pages14
JournalPlant Molecular Biology Reporter
Volume33
Issue number3
DOIs
StatePublished - Aug 14 2014
Externally publishedYes

Fingerprint

Glutaredoxins
Salicylic Acid
Arabidopsis
salicylic acid
promoter regions
Genes
genes
Genetic Promoter Regions
stress response
transcription (genetics)
exposure pathways
Physiological Stress
knockout mutants
RNA Polymerase II
DNA-directed RNA polymerase
hormones
Hormones
Gene Expression
gene expression

Keywords

  • as-1-like element
  • Glutaredoxin GRXC9 (GRX480)
  • NPR1-independent
  • Salicylic acid
  • TGA transcription factors

ASJC Scopus subject areas

  • Plant Science
  • Molecular Biology

Cite this

Herrera-Vásquez, A., Carvallo, L., Blanco, F., Tobar, M., Villarroel-Candia, E., Vicente-Carbajosa, J., ... Holuigue, L. (2014). Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis. Plant Molecular Biology Reporter, 33(3), 624-637. https://doi.org/10.1007/s11105-014-0782-5

Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis. / Herrera-Vásquez, Ariel; Carvallo, Loreto; Blanco, Francisca; Tobar, Mariola; Villarroel-Candia, Eva; Vicente-Carbajosa, Jesús; Salinas, Paula; Holuigue, Loreto.

In: Plant Molecular Biology Reporter, Vol. 33, No. 3, 14.08.2014, p. 624-637.

Research output: Contribution to journalArticle

Herrera-Vásquez, A, Carvallo, L, Blanco, F, Tobar, M, Villarroel-Candia, E, Vicente-Carbajosa, J, Salinas, P & Holuigue, L 2014, 'Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis', Plant Molecular Biology Reporter, vol. 33, no. 3, pp. 624-637. https://doi.org/10.1007/s11105-014-0782-5
Herrera-Vásquez, Ariel ; Carvallo, Loreto ; Blanco, Francisca ; Tobar, Mariola ; Villarroel-Candia, Eva ; Vicente-Carbajosa, Jesús ; Salinas, Paula ; Holuigue, Loreto. / Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis. In: Plant Molecular Biology Reporter. 2014 ; Vol. 33, No. 3. pp. 624-637.
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AU - Villarroel-Candia, Eva

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