Tracking surface glycans on live cancer cells with single-molecule sensitivity

Hao Jiang, Brian P. English, Rachel Hazan, Peng Wu, Ben Ovryn

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Using a combination of metabolically labeled glycans, a bioorthogonal copper(I)-catalyzed azide-alkyne cycloaddition, and the controlled bleaching of fluorescent probes conjugated to azide- or alkyne-tagged glycans, a sufficiently low spatial density of dye-labeled glycans was achieved, enabling dynamic single-molecule tracking and super-resolution imaging of N-linked sialic acids and O-linked N-acetyl galactosamine (GalNAc) on the membrane of live cells. Analysis of the trajectories of these dye-labeled glycans in mammary cancer cells revealed constrained diffusion of both N- and O-linked glycans, which was interpreted as reflecting the mobility of the glycan rather than to be caused by transient immobilization owing to spatial inhomogeneities on the plasma membrane. Stochastic optical reconstruction microscopy (STORM) imaging revealed the structure of dynamic membrane nanotubes.

Original languageEnglish (US)
Pages (from-to)1765-1769
Number of pages5
JournalAngewandte Chemie - International Edition
Volume54
Issue number6
DOIs
StatePublished - Feb 9 2015

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Polysaccharides
Galactosamine
Dyes
Cells
Membranes
Imaging techniques
Molecules
Cycloaddition
Cell membranes
Bleaching
Nanotubes
Microscopic examination
Alkynes
Azides
Trajectories
Copper
Acids
Coloring Agents
Sialic Acids
Fluorescent Dyes

Keywords

  • Click chemistry
  • Glycans
  • Membrane proteins
  • Single-molecule studies
  • Stochastic optical reconstruction microscopy (storm)

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

Cite this

Tracking surface glycans on live cancer cells with single-molecule sensitivity. / Jiang, Hao; English, Brian P.; Hazan, Rachel; Wu, Peng; Ovryn, Ben.

In: Angewandte Chemie - International Edition, Vol. 54, No. 6, 09.02.2015, p. 1765-1769.

Research output: Contribution to journalArticle

Jiang, Hao ; English, Brian P. ; Hazan, Rachel ; Wu, Peng ; Ovryn, Ben. / Tracking surface glycans on live cancer cells with single-molecule sensitivity. In: Angewandte Chemie - International Edition. 2015 ; Vol. 54, No. 6. pp. 1765-1769.
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