Tracking N-acetyllactosamine on cell-surface glycans in vivo

Tianqing Zheng, Hao Jiang, Marilyn Gros, David Soriano Del Amo, Subha Sundaram, Gregoire Lauvau, Florence Marlow, Yi Liu, Pamela Stanley, Peng Wu

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

In living color: Many mammalian glycans associated with signaling receptors contain terminal or penultimate N-acetyllactosamine. A highly specific method for labeling this disaccharide on cell-surface glycoproteins of live cultured cells and zebrafish embryos is reported. The two-step chemoenzymatic approach involves in situ fucosylation followed by a bioorthogonal click reaction (see scheme; α(1,3)FucT=α(1,3)-fucosyltransferase).

Original languageEnglish (US)
Pages (from-to)4113-4118
Number of pages6
JournalAngewandte Chemie - International Edition
Volume50
Issue number18
DOIs
StatePublished - Apr 26 2011

Fingerprint

galactoside 3-fucosyltransferase
Glycoproteins
Disaccharides
Membrane Glycoproteins
Labeling
Polysaccharides
Cells
Color
N-acetyllactosamine

Keywords

  • click chemistry
  • enzyme catalysis
  • glycoconjugates
  • glycosylation
  • transferases

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

Cite this

Zheng, T., Jiang, H., Gros, M., Soriano Del Amo, D., Sundaram, S., Lauvau, G., ... Wu, P. (2011). Tracking N-acetyllactosamine on cell-surface glycans in vivo. Angewandte Chemie - International Edition, 50(18), 4113-4118. https://doi.org/10.1002/anie.201100265

Tracking N-acetyllactosamine on cell-surface glycans in vivo. / Zheng, Tianqing; Jiang, Hao; Gros, Marilyn; Soriano Del Amo, David; Sundaram, Subha; Lauvau, Gregoire; Marlow, Florence; Liu, Yi; Stanley, Pamela; Wu, Peng.

In: Angewandte Chemie - International Edition, Vol. 50, No. 18, 26.04.2011, p. 4113-4118.

Research output: Contribution to journalArticle

Zheng, T, Jiang, H, Gros, M, Soriano Del Amo, D, Sundaram, S, Lauvau, G, Marlow, F, Liu, Y, Stanley, P & Wu, P 2011, 'Tracking N-acetyllactosamine on cell-surface glycans in vivo', Angewandte Chemie - International Edition, vol. 50, no. 18, pp. 4113-4118. https://doi.org/10.1002/anie.201100265
Zheng, Tianqing ; Jiang, Hao ; Gros, Marilyn ; Soriano Del Amo, David ; Sundaram, Subha ; Lauvau, Gregoire ; Marlow, Florence ; Liu, Yi ; Stanley, Pamela ; Wu, Peng. / Tracking N-acetyllactosamine on cell-surface glycans in vivo. In: Angewandte Chemie - International Edition. 2011 ; Vol. 50, No. 18. pp. 4113-4118.
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