Tracking N-acetyllactosamine on cell-surface glycans in vivo

Tianqing Zheng; Hao Jiang; Marilyn Gros; David Soriano Del Amo; Subha Sundaram; Grégoire Lauvau; Florence Marlow; Yi Liu; Pamela Stanley; Peng Wu

doi:10.1002/anie.201100265

Tracking N-acetyllactosamine on cell-surface glycans in vivo

Tianqing Zheng, Hao Jiang, Marilyn Gros, David Soriano Del Amo, Subha Sundaram, Grégoire Lauvau, Florence Marlow, Yi Liu, Pamela Stanley, Peng Wu

Research output: Contribution to journal › Article › peer-review

71 Scopus citations

Abstract

In living color: Many mammalian glycans associated with signaling receptors contain terminal or penultimate N-acetyllactosamine. A highly specific method for labeling this disaccharide on cell-surface glycoproteins of live cultured cells and zebrafish embryos is reported. The two-step chemoenzymatic approach involves in situ fucosylation followed by a bioorthogonal click reaction (see scheme; α(1,3)FucT=α(1,3)-fucosyltransferase).

Original language	English (US)
Pages (from-to)	4113-4118
Number of pages	6
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	18
DOIs	https://doi.org/10.1002/anie.201100265
State	Published - Apr 26 2011

Keywords

click chemistry
enzyme catalysis
glycoconjugates
glycosylation
transferases

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.201100265

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title = "Tracking N-acetyllactosamine on cell-surface glycans in vivo",

abstract = "In living color: Many mammalian glycans associated with signaling receptors contain terminal or penultimate N-acetyllactosamine. A highly specific method for labeling this disaccharide on cell-surface glycoproteins of live cultured cells and zebrafish embryos is reported. The two-step chemoenzymatic approach involves in situ fucosylation followed by a bioorthogonal click reaction (see scheme; α(1,3)FucT=α(1,3)-fucosyltransferase).",

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T1 - Tracking N-acetyllactosamine on cell-surface glycans in vivo

AU - Zheng, Tianqing

AU - Jiang, Hao

AU - Gros, Marilyn

AU - Soriano Del Amo, David

AU - Sundaram, Subha

AU - Lauvau, Grégoire

AU - Marlow, Florence

AU - Liu, Yi

AU - Stanley, Pamela

AU - Wu, Peng

PY - 2011/4/26

Y1 - 2011/4/26

N2 - In living color: Many mammalian glycans associated with signaling receptors contain terminal or penultimate N-acetyllactosamine. A highly specific method for labeling this disaccharide on cell-surface glycoproteins of live cultured cells and zebrafish embryos is reported. The two-step chemoenzymatic approach involves in situ fucosylation followed by a bioorthogonal click reaction (see scheme; α(1,3)FucT=α(1,3)-fucosyltransferase).

AB - In living color: Many mammalian glycans associated with signaling receptors contain terminal or penultimate N-acetyllactosamine. A highly specific method for labeling this disaccharide on cell-surface glycoproteins of live cultured cells and zebrafish embryos is reported. The two-step chemoenzymatic approach involves in situ fucosylation followed by a bioorthogonal click reaction (see scheme; α(1,3)FucT=α(1,3)-fucosyltransferase).

KW - click chemistry

KW - enzyme catalysis

KW - glycoconjugates

KW - glycosylation

KW - transferases

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Tracking N-acetyllactosamine on cell-surface glycans in vivo

Abstract

Keywords

ASJC Scopus subject areas

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