Tracking N-acetyllactosamine on cell-surface glycans in vivo

Tianqing Zheng, Hao Jiang, Marilyn Gros, David Soriano Del Amo, Subha Sundaram, Grégoire Lauvau, Florence Marlow, Yi Liu, Pamela Stanley, Peng Wu

51 Scopus citations

Abstract

In living color: Many mammalian glycans associated with signaling receptors contain terminal or penultimate N-acetyllactosamine. A highly specific method for labeling this disaccharide on cell-surface glycoproteins of live cultured cells and zebrafish embryos is reported. The two-step chemoenzymatic approach involves in situ fucosylation followed by a bioorthogonal click reaction (see scheme; α(1,3)FucT=α(1,3)-fucosyltransferase).

Original languageEnglish (US)
Pages (from-to)4113-4118
Number of pages6
JournalAngewandte Chemie - International Edition
Volume50
Issue number18
DOIs
StatePublished - Apr 26 2011

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Keywords

  • click chemistry
  • enzyme catalysis
  • glycoconjugates
  • glycosylation
  • transferases

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

Cite this

Zheng, T., Jiang, H., Gros, M., Soriano Del Amo, D., Sundaram, S., Lauvau, G., Marlow, F., Liu, Y., Stanley, P., & Wu, P. (2011). Tracking N-acetyllactosamine on cell-surface glycans in vivo. Angewandte Chemie - International Edition, 50(18), 4113-4118. https://doi.org/10.1002/anie.201100265