Tracking N-acetyllactosamine on cell-surface glycans in vivo

Tianqing Zheng, Hao Jiang, Marilyn Gros, David Soriano Del Amo, Subha Sundaram, Grégoire Lauvau, Florence Marlow, Yi Liu, Pamela Stanley, Peng Wu

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

In living color: Many mammalian glycans associated with signaling receptors contain terminal or penultimate N-acetyllactosamine. A highly specific method for labeling this disaccharide on cell-surface glycoproteins of live cultured cells and zebrafish embryos is reported. The two-step chemoenzymatic approach involves in situ fucosylation followed by a bioorthogonal click reaction (see scheme; α(1,3)FucT=α(1,3)-fucosyltransferase).

Original languageEnglish (US)
Pages (from-to)4113-4118
Number of pages6
JournalAngewandte Chemie - International Edition
Volume50
Issue number18
DOIs
StatePublished - Apr 26 2011

Keywords

  • click chemistry
  • enzyme catalysis
  • glycoconjugates
  • glycosylation
  • transferases

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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