In living color: Many mammalian glycans associated with signaling receptors contain terminal or penultimate N-acetyllactosamine. A highly specific method for labeling this disaccharide on cell-surface glycoproteins of live cultured cells and zebrafish embryos is reported. The two-step chemoenzymatic approach involves in situ fucosylation followed by a bioorthogonal click reaction (see scheme; α(1,3)FucT=α(1,3)-fucosyltransferase).
|Original language||English (US)|
|Number of pages||6|
|Journal||Angewandte Chemie - International Edition|
|State||Published - Apr 26 2011|
- click chemistry
- enzyme catalysis
ASJC Scopus subject areas