Toxicity of free radicals to mesothelial cells and peritoneal membrane

A. Breborowicz, J. Witowski, K. Wieczorowska, L. Martis, K. D. Serkes, D. G. Oreopoulos

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We studied the toxicity of free radicals to human mesothelial cells in vitro and to the peritoneal membrane of rats during peritoneal dialysis. Free radicals cause damage to mesothelial cells as measured by release of cytosolic markers such as 86Rb and lactate dehydrogenase. Vitamin E neutralized the toxic effect of free radicals in vitro. Human mesothelial cells exposed over 6 h to a mixture of essential and nonessential amino acids in medium are more vulnerable to the cytotoxic effect of free radicals than control cells exposed to medium alone. Cells exposed previously to glucose or glycerol are less vulnerable than controls. In rats free radicals generated intraperitoneally by a xanthine-xanthine oxidase system induce changes in peritoneal permeability similar to those observed during peritonitis: loss of ultrafiltration, increased glucose absorption from the dialysate and augmented transperitoneal loss of albumin. In addition lipids in the peritoneum became perixodated. The addition of vitamin E to the peritoneal fluid with xanthine-xanthine oxidase prevents peroxidation of lipids and the subsequent loss of ultrafiltration. Our results show that free radicals may exert a potentially toxic effect on the peritoneal membrane during peritonitis. In such circumstances the addition of free radical scavenger to the dialysis fluid may preserve intact structure and function of peritoneum.

Original languageEnglish (US)
Pages (from-to)62-66
Number of pages5
JournalNephron
Volume65
Issue number1
StatePublished - 1993
Externally publishedYes

Fingerprint

Free Radicals
Cell Membrane
Xanthine
Xanthine Oxidase
Poisons
Peritoneum
Ultrafiltration
Peritonitis
Vitamin E
Glucose
Free Radical Scavengers
Essential Amino Acids
Membranes
Ascitic Fluid
Dialysis Solutions
Peritoneal Dialysis
L-Lactate Dehydrogenase
Glycerol
Lipid Peroxidation
Dialysis

Keywords

  • Human
  • Mesothelial cells
  • Oxygen-free radicals
  • Peritoneal membrane

ASJC Scopus subject areas

  • Nephrology

Cite this

Breborowicz, A., Witowski, J., Wieczorowska, K., Martis, L., Serkes, K. D., & Oreopoulos, D. G. (1993). Toxicity of free radicals to mesothelial cells and peritoneal membrane. Nephron, 65(1), 62-66.

Toxicity of free radicals to mesothelial cells and peritoneal membrane. / Breborowicz, A.; Witowski, J.; Wieczorowska, K.; Martis, L.; Serkes, K. D.; Oreopoulos, D. G.

In: Nephron, Vol. 65, No. 1, 1993, p. 62-66.

Research output: Contribution to journalArticle

Breborowicz, A, Witowski, J, Wieczorowska, K, Martis, L, Serkes, KD & Oreopoulos, DG 1993, 'Toxicity of free radicals to mesothelial cells and peritoneal membrane', Nephron, vol. 65, no. 1, pp. 62-66.
Breborowicz A, Witowski J, Wieczorowska K, Martis L, Serkes KD, Oreopoulos DG. Toxicity of free radicals to mesothelial cells and peritoneal membrane. Nephron. 1993;65(1):62-66.
Breborowicz, A. ; Witowski, J. ; Wieczorowska, K. ; Martis, L. ; Serkes, K. D. ; Oreopoulos, D. G. / Toxicity of free radicals to mesothelial cells and peritoneal membrane. In: Nephron. 1993 ; Vol. 65, No. 1. pp. 62-66.
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