Toxic effect of methamphetamine on the retina of CD1 mice

Hong Lai, Huiyang Zeng, Cheng Zhang, Lin Wang, Mark O M Tso, Shenghan Lai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose: To investigate whether systemic administration of methamphetamine (METH) induces retinal damage in CD1 mice. Materials and Methods: Eighteen male CD1 mice were randomly assigned to three groups, six mice per group: Group 1 receiving a single dose of 40 mg/kg METH, Group 2 receiving four doses of 10 mg/kg METH, and Group 3 (control) receiving 40 mg/kg 0.9 NaCl solution. METH and NaCl were administered by intraperitoneal injection. Immunostaining of glial fibrillary acidic protein (GFAP), S-100 for astrocytes and Muller cells, CD11b for microglia, and tyrosine hydroxylase (TH) and TUNEL labeling for apoptotic cell death were performed on the retinal sections on day 1 and day 7 post-exposure. Results: GFAP and S-100 immunoreactivity was observed in Group 1 mice. CD11b cells in Group 1 mice showed more intensely stained shorter and thicker cellular processes than Groups 2 and 3, indicating activated microglia in the mice exposed to large-dose METH. No significant difference in TH level was seen among the three groups. TUNEL labeling did not reveal positive cells in the retinas of any of the 18 CD1 mice. Conclusions: A single large dose of METH induces an increase in short-term protein expression of GFAP and S-100 and in microglial activation. The results suggest that METH has a neurotoxic effect on CD1 mouse retina.

Original languageEnglish (US)
Pages (from-to)785-790
Number of pages6
JournalCurrent Eye Research
Volume34
Issue number9
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Methamphetamine
Poisons
Retina
Glial Fibrillary Acidic Protein
Protein S
In Situ Nick-End Labeling
Tyrosine 3-Monooxygenase
Microglia
Ependymoglial Cells
Group Processes
Intraperitoneal Injections
Astrocytes
Cell Death
Control Groups

Keywords

  • CD1 mice
  • Methamphetamine
  • Neurotoxic effect
  • Retinal damage

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Toxic effect of methamphetamine on the retina of CD1 mice. / Lai, Hong; Zeng, Huiyang; Zhang, Cheng; Wang, Lin; Tso, Mark O M; Lai, Shenghan.

In: Current Eye Research, Vol. 34, No. 9, 2009, p. 785-790.

Research output: Contribution to journalArticle

Lai, Hong ; Zeng, Huiyang ; Zhang, Cheng ; Wang, Lin ; Tso, Mark O M ; Lai, Shenghan. / Toxic effect of methamphetamine on the retina of CD1 mice. In: Current Eye Research. 2009 ; Vol. 34, No. 9. pp. 785-790.
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AB - Purpose: To investigate whether systemic administration of methamphetamine (METH) induces retinal damage in CD1 mice. Materials and Methods: Eighteen male CD1 mice were randomly assigned to three groups, six mice per group: Group 1 receiving a single dose of 40 mg/kg METH, Group 2 receiving four doses of 10 mg/kg METH, and Group 3 (control) receiving 40 mg/kg 0.9 NaCl solution. METH and NaCl were administered by intraperitoneal injection. Immunostaining of glial fibrillary acidic protein (GFAP), S-100 for astrocytes and Muller cells, CD11b for microglia, and tyrosine hydroxylase (TH) and TUNEL labeling for apoptotic cell death were performed on the retinal sections on day 1 and day 7 post-exposure. Results: GFAP and S-100 immunoreactivity was observed in Group 1 mice. CD11b cells in Group 1 mice showed more intensely stained shorter and thicker cellular processes than Groups 2 and 3, indicating activated microglia in the mice exposed to large-dose METH. No significant difference in TH level was seen among the three groups. TUNEL labeling did not reveal positive cells in the retinas of any of the 18 CD1 mice. Conclusions: A single large dose of METH induces an increase in short-term protein expression of GFAP and S-100 and in microglial activation. The results suggest that METH has a neurotoxic effect on CD1 mouse retina.

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