TNPO3 is required for HIV-1 replication after nuclear import but prior to integration and binds the HIV-1 core

Jose Carlos Valle-Casuso, Francesca Di Nunzio, Yang Yang, Natalia Reszka, Maritza Lienlaf, Nathalie Arhel, Patricio Perez, Abraham L. Brass, Felipe Diaz-Griffero

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

TNPO3 is a nuclear importer required for HIV-1 infection. Here, we show that depletion of TNPO3 leads to an HIV-1 block after nuclear import but prior to integration. To investigate the mechanistic requirement of TNPO3 in HIV-1 infection, we tested the binding of TNPO3 to the HIV-1 core and found that TNPO3 binds to the HIV-1 core. Overall, this work suggests that TNPO3 interacts with the incoming HIV-1 core in the cytoplasm to assist a process that is important for HIV-1 infection after nuclear import.

Original languageEnglish (US)
Pages (from-to)5931-5936
Number of pages6
JournalJournal of Virology
Volume86
Issue number10
DOIs
StatePublished - May 2012

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Cell Nucleus Active Transport
Human immunodeficiency virus 1
imports
HIV-1
HIV Infections
infection
Cytoplasm
cytoplasm

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

TNPO3 is required for HIV-1 replication after nuclear import but prior to integration and binds the HIV-1 core. / Valle-Casuso, Jose Carlos; Di Nunzio, Francesca; Yang, Yang; Reszka, Natalia; Lienlaf, Maritza; Arhel, Nathalie; Perez, Patricio; Brass, Abraham L.; Diaz-Griffero, Felipe.

In: Journal of Virology, Vol. 86, No. 10, 05.2012, p. 5931-5936.

Research output: Contribution to journalArticle

Valle-Casuso, JC, Di Nunzio, F, Yang, Y, Reszka, N, Lienlaf, M, Arhel, N, Perez, P, Brass, AL & Diaz-Griffero, F 2012, 'TNPO3 is required for HIV-1 replication after nuclear import but prior to integration and binds the HIV-1 core', Journal of Virology, vol. 86, no. 10, pp. 5931-5936. https://doi.org/10.1128/JVI.00451-12
Valle-Casuso, Jose Carlos ; Di Nunzio, Francesca ; Yang, Yang ; Reszka, Natalia ; Lienlaf, Maritza ; Arhel, Nathalie ; Perez, Patricio ; Brass, Abraham L. ; Diaz-Griffero, Felipe. / TNPO3 is required for HIV-1 replication after nuclear import but prior to integration and binds the HIV-1 core. In: Journal of Virology. 2012 ; Vol. 86, No. 10. pp. 5931-5936.
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