TNPO3 is required for HIV-1 replication after nuclear import but prior to integration and binds the HIV-1 core

Jose Carlos Valle-Casuso, Francesca Di Nunzio, Yang Yang, Natalia Reszka, Maritza Lienlaf, Nathalie Arhel, Patricio Perez, Abraham L. Brass, Felipe Diaz-Griffero

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

TNPO3 is a nuclear importer required for HIV-1 infection. Here, we show that depletion of TNPO3 leads to an HIV-1 block after nuclear import but prior to integration. To investigate the mechanistic requirement of TNPO3 in HIV-1 infection, we tested the binding of TNPO3 to the HIV-1 core and found that TNPO3 binds to the HIV-1 core. Overall, this work suggests that TNPO3 interacts with the incoming HIV-1 core in the cytoplasm to assist a process that is important for HIV-1 infection after nuclear import.

Original languageEnglish (US)
Pages (from-to)5931-5936
Number of pages6
JournalJournal of virology
Volume86
Issue number10
DOIs
StatePublished - May 2012

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Valle-Casuso, J. C., Di Nunzio, F., Yang, Y., Reszka, N., Lienlaf, M., Arhel, N., Perez, P., Brass, A. L., & Diaz-Griffero, F. (2012). TNPO3 is required for HIV-1 replication after nuclear import but prior to integration and binds the HIV-1 core. Journal of virology, 86(10), 5931-5936. https://doi.org/10.1128/JVI.00451-12