TLR4 and toll-IL-1 receptor domain-containing adapter-inducing IFN-β, but not MyD88, regulate Escherichia coli-induced dendritic cell maturation and apoptosis in vivo

Carl De Trez, Bernard Pajak, Maryse Brait, Nicolas Glaichenhaus, Jacques Urbain, Muriel Moser, Gregoire Lauvau, Eric Muraille

Research output: Contribution to journalArticle

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Abstract

Dendritic cells (DC) are short-lived, professional APCs that play a central role in the generation of adaptive immune responses. Induction of efficient immune responses is dependent on how long DCs survive in the host. Therefore, the regulation of DC apoptosis in vivo during infection remains an important question that requires further investigation. The impact of Escherichia coli bacteremia on DCs has never been analyzed. We show here that i.v. or i.p. administration of live or heat-killed E. coli in mice induces splenic DC migration, maturation, and apoptosis. We further characterize which TLR and Toll-IL-IR (TIR)-containing adaptor molecules regulate these processes in vivo. In this model, DC maturation is impaired in TLR2-/-, TLR4 -/- and TIR domain-containing adapter-inducing IFN-β (TRIF) -/- mice. In contrast, DC apoptosis is reduced only in TLR4 -/- and TRIF-/- mice. As expected, DC apoptosis induced by the TLR4 ligand LPS is also abolished in these mice. Injection of the TLR9 ligand CpG-oligodeoxynucleotide (synthetic bacterial DNA) induces DC migration and maturation, but only modest DC apoptosis when compared with LPS and E. coli. Together, these results suggest that E. coli bacteremia directly impacts on DC maturation and survival in vivo through a TLR4-TRIF-dependent signaling pathway.

Original languageEnglish (US)
Pages (from-to)839-846
Number of pages8
JournalJournal of Immunology
Volume175
Issue number2
StatePublished - Jul 15 2005
Externally publishedYes

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Interleukin-1 Receptors
Dendritic Cells
Apoptosis
Escherichia coli
Bacteremia
Cell Movement
Ligands
Bacterial DNA
Oligodeoxyribonucleotides
Adaptive Immunity
Cell Survival
Hot Temperature
Injections

ASJC Scopus subject areas

  • Immunology

Cite this

TLR4 and toll-IL-1 receptor domain-containing adapter-inducing IFN-β, but not MyD88, regulate Escherichia coli-induced dendritic cell maturation and apoptosis in vivo. / De Trez, Carl; Pajak, Bernard; Brait, Maryse; Glaichenhaus, Nicolas; Urbain, Jacques; Moser, Muriel; Lauvau, Gregoire; Muraille, Eric.

In: Journal of Immunology, Vol. 175, No. 2, 15.07.2005, p. 839-846.

Research output: Contribution to journalArticle

De Trez, Carl ; Pajak, Bernard ; Brait, Maryse ; Glaichenhaus, Nicolas ; Urbain, Jacques ; Moser, Muriel ; Lauvau, Gregoire ; Muraille, Eric. / TLR4 and toll-IL-1 receptor domain-containing adapter-inducing IFN-β, but not MyD88, regulate Escherichia coli-induced dendritic cell maturation and apoptosis in vivo. In: Journal of Immunology. 2005 ; Vol. 175, No. 2. pp. 839-846.
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abstract = "Dendritic cells (DC) are short-lived, professional APCs that play a central role in the generation of adaptive immune responses. Induction of efficient immune responses is dependent on how long DCs survive in the host. Therefore, the regulation of DC apoptosis in vivo during infection remains an important question that requires further investigation. The impact of Escherichia coli bacteremia on DCs has never been analyzed. We show here that i.v. or i.p. administration of live or heat-killed E. coli in mice induces splenic DC migration, maturation, and apoptosis. We further characterize which TLR and Toll-IL-IR (TIR)-containing adaptor molecules regulate these processes in vivo. In this model, DC maturation is impaired in TLR2-/-, TLR4 -/- and TIR domain-containing adapter-inducing IFN-β (TRIF) -/- mice. In contrast, DC apoptosis is reduced only in TLR4 -/- and TRIF-/- mice. As expected, DC apoptosis induced by the TLR4 ligand LPS is also abolished in these mice. Injection of the TLR9 ligand CpG-oligodeoxynucleotide (synthetic bacterial DNA) induces DC migration and maturation, but only modest DC apoptosis when compared with LPS and E. coli. Together, these results suggest that E. coli bacteremia directly impacts on DC maturation and survival in vivo through a TLR4-TRIF-dependent signaling pathway.",
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AU - De Trez, Carl

AU - Pajak, Bernard

AU - Brait, Maryse

AU - Glaichenhaus, Nicolas

AU - Urbain, Jacques

AU - Moser, Muriel

AU - Lauvau, Gregoire

AU - Muraille, Eric

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