Three kinds of current in response to substance P in bullfrog DRG neurons

Yunlei Yang, Kai Hui Yao, Yuan Zheng Gu, Bing Cai Guan, Zhi Wang Li

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In response to SP applied externally, neurons freshly isolated from bullfrog dorsal root ganglion (DRG) showed three kinds of current (I SP), i.e. slow, fast and moderately activating ISPs. All the three kinds of ISP were inward currents, and were completely blocked by either peptide antagonist of SP receptor spantide or non-peptide antagonist of SP receptor WIN51708. The slow activating ISP showed slow kinetic features. Replacement of NaCl in external solution by NMDG had no effect on this kind of ISP, while Ba2+ abolished it almost completely, thus the ionic mechanism underlying slow activating I SP was deduced to be the closure of K+ channels. The fast activating ISP in bullfrog DRG neurons, just as in rat DRG neurons, was proved to be caused by the opening of Na+ preferring non-selective cation channel, for it was abolished almost completely by replacement of NaCl in external solution with equimolar NMDG. The moderately activating ISP was similar to the fast activating ISP in current configuration, however, its kinetic characteristics lay between those of fast and slow activating ISPs. Either NMDG or Ba2+ suppressed this kind of ISP partially. Therefore the moderately activating ISP might be mediated by non-selective cation channel. We used repatch technique to explore the intracellular mechanism underlying the three kinds of ISP and found that the three kinds of ISP were caused by the activity of either G-protein coupled channel (slow activating ISP) or directly opened channel (fast activating I SP) or both (moderately activating ISP ).

Original languageEnglish (US)
Pages (from-to)70-77
Number of pages8
JournalBrain Research
Volume981
Issue number1-2
DOIs
StatePublished - Aug 15 2003
Externally publishedYes

Fingerprint

Rana catesbeiana
Spinal Ganglia
Substance P
Neurons
Cations
GTP-Binding Proteins
Peptides

Keywords

  • Bullfrog
  • Current
  • Dorsal root ganglion neuron
  • Substance P

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Three kinds of current in response to substance P in bullfrog DRG neurons. / Yang, Yunlei; Yao, Kai Hui; Gu, Yuan Zheng; Guan, Bing Cai; Li, Zhi Wang.

In: Brain Research, Vol. 981, No. 1-2, 15.08.2003, p. 70-77.

Research output: Contribution to journalArticle

Yang, Yunlei ; Yao, Kai Hui ; Gu, Yuan Zheng ; Guan, Bing Cai ; Li, Zhi Wang. / Three kinds of current in response to substance P in bullfrog DRG neurons. In: Brain Research. 2003 ; Vol. 981, No. 1-2. pp. 70-77.
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