Three-dimensional organization of rat hepatocyte cytoskeleton

Relation to the asialoglycoprotein endocytosis pathway

P. M. Novikoff, M. Cammer, L. Tao, H. Oda, R. J. Stockert, Allan W. Wolkoff, P. Satir

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Analysis by confocal microscopy has revealed features of the microtubule network of rat hepatocytes in culture, establishing the three-dimensional disposition of the microtubule-based cytoskeleton, its relation to the actin-based cytoskeleton and to ligand-containing endosomes during receptor-mediated endocytosis and the alterations in its structure and disposition by the microtubule pertubant, Taxol. By co-localization studies, we have been able to demonstrate that the microtubules have a significant role in receptor-mediated endocytosis of asialoglycoproteins in this cell. Asialoorosomucoid-containing endosomes attach to widely spaced arrays of microtubules running under the base-lateral surface of the hepatocytes 5-15 minutes after the initiation of endocytosis and then travel along microtubule paths to become concentrated with microtubules near the centrosome and at bile canaliculi after 30-60 minutes of receptor-mediated endocytosis. Receptor-mediated endocytosis is affected, but not abolished by Taxol, which inhibits the rate of asialoorosomucoid degradation at the same concentrations as those that disrupt microtubule and cytoplasmic dynein distribution, and that prevent the concentration of endosomes centrally. The results support suggestions that asialoorosomucoid-containing endosomes are captured by microtubules just below the actin layer at the cell periphery and these are actively transported centrally along microtubules, possibly by cytoplasmic dynein, so that the concentration of endosomes near the centrosome, and the subsequent efficient lysosomal degradation of ligand, are consequences of the confluence of microtubules in this region.

Original languageEnglish (US)
Pages (from-to)21-32
Number of pages12
JournalJournal of Cell Science
Volume109
Issue number1
StatePublished - Jan 1996

Fingerprint

Asialoglycoproteins
Endocytosis
Cytoskeleton
Microtubules
Hepatocytes
Endosomes
Cytoplasmic Dyneins
Centrosome
Paclitaxel
Bile Canaliculi
Ligands
Actin Cytoskeleton
Confocal Microscopy

Keywords

  • Actin
  • Cell polarity
  • Centriole
  • Dynein
  • Microtubule
  • Microtubule organizing center
  • Receptor-mediated endocytosis
  • Taxol
  • Tubulin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Three-dimensional organization of rat hepatocyte cytoskeleton : Relation to the asialoglycoprotein endocytosis pathway. / Novikoff, P. M.; Cammer, M.; Tao, L.; Oda, H.; Stockert, R. J.; Wolkoff, Allan W.; Satir, P.

In: Journal of Cell Science, Vol. 109, No. 1, 01.1996, p. 21-32.

Research output: Contribution to journalArticle

Novikoff, PM, Cammer, M, Tao, L, Oda, H, Stockert, RJ, Wolkoff, AW & Satir, P 1996, 'Three-dimensional organization of rat hepatocyte cytoskeleton: Relation to the asialoglycoprotein endocytosis pathway', Journal of Cell Science, vol. 109, no. 1, pp. 21-32.
Novikoff, P. M. ; Cammer, M. ; Tao, L. ; Oda, H. ; Stockert, R. J. ; Wolkoff, Allan W. ; Satir, P. / Three-dimensional organization of rat hepatocyte cytoskeleton : Relation to the asialoglycoprotein endocytosis pathway. In: Journal of Cell Science. 1996 ; Vol. 109, No. 1. pp. 21-32.
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