The use of chitosan to damage Cryptococcus neoformans biofilms

Luis R. Martinez, Mircea Radu Mihu, George Han, Susana Frases, Radames J B Cordero, Arturo Casadevall, Adam J. Friedman, Joel M. Friedman, Joshua D. Nosanchuk

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The use of indwelling medical devices (e.g. pacemakers, prosthetic joints, catheters, etc) continues to increase, yet these devices are all too often complicated by infections with biofilm-forming microbes with increased resistance to antimicrobial agents and host defense mechanisms. We investigated the ability of chitosan, a polymer isolated from crustacean exoskeletons, to damage biofilms formed by the pathogenic fungus Cryptococcus neoformans. Using 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium-hydroxide (XTT) reduction assay and CFU determinations, we showed that chitosan significantly reduced both the metabolic activity of the biofilms and cell viability, respectively. We further demonstrated that chitosan penetrated biofilms and damaged fungal cells using confocal and scanning electron microscopy. Notably, melanization, an important virulence determinant of C. neoformans, did not protect cryptococcal biofilms against chitosan. The chitosan concentrations used in this study to evaluate fungal biofilm susceptibility were not toxic to human endothelial cells. Our results indicate that cryptococcal biofilms are susceptible to treatment with chitosan, suggesting an option for the prevention or treatment of fungal biofilms on indwelling medical devices.

Original languageEnglish (US)
Pages (from-to)669-679
Number of pages11
JournalBiomaterials
Volume31
Issue number4
DOIs
StatePublished - 2010

Fingerprint

Cryptococcus neoformans
Chitosan
Biofilms
Equipment and Supplies
Antimicrobial agents
Pacemakers
Catheters
Poisons
Endothelial cells
Anti-Infective Agents
Prosthetics
Fungi
Electron Scanning Microscopy
Virulence
Assays
Cell Survival
Polymers
Endothelial Cells
Joints
Cells

Keywords

  • Biofilms
  • Chitosan
  • Cryptococcus neoformans
  • Fungi
  • Melanin
  • Planktonic

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Martinez, L. R., Mihu, M. R., Han, G., Frases, S., Cordero, R. J. B., Casadevall, A., ... Nosanchuk, J. D. (2010). The use of chitosan to damage Cryptococcus neoformans biofilms. Biomaterials, 31(4), 669-679. https://doi.org/10.1016/j.biomaterials.2009.09.087

The use of chitosan to damage Cryptococcus neoformans biofilms. / Martinez, Luis R.; Mihu, Mircea Radu; Han, George; Frases, Susana; Cordero, Radames J B; Casadevall, Arturo; Friedman, Adam J.; Friedman, Joel M.; Nosanchuk, Joshua D.

In: Biomaterials, Vol. 31, No. 4, 2010, p. 669-679.

Research output: Contribution to journalArticle

Martinez, LR, Mihu, MR, Han, G, Frases, S, Cordero, RJB, Casadevall, A, Friedman, AJ, Friedman, JM & Nosanchuk, JD 2010, 'The use of chitosan to damage Cryptococcus neoformans biofilms', Biomaterials, vol. 31, no. 4, pp. 669-679. https://doi.org/10.1016/j.biomaterials.2009.09.087
Martinez LR, Mihu MR, Han G, Frases S, Cordero RJB, Casadevall A et al. The use of chitosan to damage Cryptococcus neoformans biofilms. Biomaterials. 2010;31(4):669-679. https://doi.org/10.1016/j.biomaterials.2009.09.087
Martinez, Luis R. ; Mihu, Mircea Radu ; Han, George ; Frases, Susana ; Cordero, Radames J B ; Casadevall, Arturo ; Friedman, Adam J. ; Friedman, Joel M. ; Nosanchuk, Joshua D. / The use of chitosan to damage Cryptococcus neoformans biofilms. In: Biomaterials. 2010 ; Vol. 31, No. 4. pp. 669-679.
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