The sup8 tRNALeu gene of Schizosaccharomyces pombe has an unusual intervening sequence and reduced pairing in the anticodon stem

Martin Sumner-Smith, Herbert Hottinger, Ian Willis, Terry L. Koch, Rene Arentzen, Dieter Söll

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

We have cloned and sequenced the wild-type and suppressor alleles of the S. pombe sup8 tRNA gene. The wild-type allele has a leucine UAA anticodon and the suppressor (sup8-e) carries the opal suppressor anticodon UCA. The gene has a 16 base pair intervening sequence that, in the RNA, is predicted to form a secondary structure which involves base pairing to the 5′, rather than the usual 3′ side of the 5′ splice site. When incubated in Saccharomyces cerevisiae cell-free extracts both alleles are efficiently transcribed, the 5′ leader and 3′ trailer sequences are removed and CCA is added to the 3′ processed end; however, the intervening sequence is not excised. This finding implies that the structural requirements of the splicing endonucleases in the two yeasts have diverged. No other tRNA genes with related sequences were detected in S. pombe DNA by hybridization, suggesting that other UUA isoacceptors may be structurally dissimilar to sup8 or that the UUA codon may be decoded by a UUG leucine isoacceptor.

Original languageEnglish (US)
Pages (from-to)447-452
Number of pages6
JournalMGG Molecular & General Genetics
Volume197
Issue number3
DOIs
StatePublished - Nov 1 1984
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'The sup8 tRNA<sup>Leu</sup> gene of Schizosaccharomyces pombe has an unusual intervening sequence and reduced pairing in the anticodon stem'. Together they form a unique fingerprint.

  • Cite this