The structure of the IgE Cε2 domain and its role in stabilizing the complex with its high-affinity receptor FcεRIα

James M. McDonnell, Rosaleen Calvert, Rebecca L. Beavil, Andrew J. Beavil, Alistair J. Henry, Brian J. Sutton, Hannah J. Gould, David Cowburn

Research output: Contribution to journalArticle

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Abstract

The stability of the complex between IgE and its high-affinity receptor, FcεRI, on mast cells is a critical factor in the allergic response. The long half-life of the complex of IgE bound to this receptor in situ (∼ 2 weeks, compared with only hours for the comparable IgG complex) contributes to the permanent sensitization of these cells and, hence, to the immediate response to allergens. Here we show that the second constant domain of IgE, Cε2, which takes the place of the flexible hinge in IgG, contributes to this long half-life. When the Cε2 domain is deleted from the IgE Fc fragment, leaving only the Cε3 and Cε4 domains (Cε3-4 fragment), the rate of dissociation from the receptor is increased by greater than I order of magnitude. We report the structure of the Cε2 domain by heteronuclear NMR spectroscopy and show by chemical shift perturbation that it interacts with FcεRIα. By sedimentation equilibrium we show that the Cε2 domain binds to the Cε3-4 fragment of IgE. These interactions of Cε2 with both FcεRIα and Cε3-4 provide a structural explanation for the exceptionally slow dissociation of the IgE-FcεRIα complex.

Original languageEnglish (US)
Pages (from-to)437-441
Number of pages5
JournalNature Structural Biology
Volume8
Issue number5
DOIs
StatePublished - 2001

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Immunoglobulin E
Half-Life
Immunoglobulin G
Biomolecular Nuclear Magnetic Resonance
Immunoglobulin Fc Fragments
Chemical shift
Hinges
Sedimentation
Mast Cells
Allergens
Nuclear magnetic resonance spectroscopy
C2 Domains
Magnetic Resonance Spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

The structure of the IgE Cε2 domain and its role in stabilizing the complex with its high-affinity receptor FcεRIα. / McDonnell, James M.; Calvert, Rosaleen; Beavil, Rebecca L.; Beavil, Andrew J.; Henry, Alistair J.; Sutton, Brian J.; Gould, Hannah J.; Cowburn, David.

In: Nature Structural Biology, Vol. 8, No. 5, 2001, p. 437-441.

Research output: Contribution to journalArticle

McDonnell, JM, Calvert, R, Beavil, RL, Beavil, AJ, Henry, AJ, Sutton, BJ, Gould, HJ & Cowburn, D 2001, 'The structure of the IgE Cε2 domain and its role in stabilizing the complex with its high-affinity receptor FcεRIα', Nature Structural Biology, vol. 8, no. 5, pp. 437-441. https://doi.org/10.1038/87603
McDonnell, James M. ; Calvert, Rosaleen ; Beavil, Rebecca L. ; Beavil, Andrew J. ; Henry, Alistair J. ; Sutton, Brian J. ; Gould, Hannah J. ; Cowburn, David. / The structure of the IgE Cε2 domain and its role in stabilizing the complex with its high-affinity receptor FcεRIα. In: Nature Structural Biology. 2001 ; Vol. 8, No. 5. pp. 437-441.
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