The specificity of human anti-cytochrome c autoantibodies that arise in autoimmune disease

M. J. Mamula, R. Jemmerson, J. A. Hardin

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Cytochromes c (cyt c) are among the best characterized model Ag because their amino acid sequences and tertiary structures are well defined. One unique aspect of cyt c as an immunogen is its ability to induce autoantibody responses in animal models, although no pathology resulting from these responses has been reported. In this study, the presence and specificity of autoantibodies to cyt c were investigated in patients with SLE and related connective tissue diseases. Anti-cyt c antibodies were found in approximately 7% of patient sera and were statistically associated with the expression of antimitochondrial antibodies but were not statistically associated with any disease subset among those represented. Anti-cyt c was not associated with the presence of autoantibodies to DNA, histones, Ro, La, or Sm autoantigens. Most of the autoantibodies were specific for native or native-like forms of cyt c but antibodies to denatured forms were also apparent. Autoantibody binding was shown to be directed predominantly at selected sites of evolutionary variability within cyt c. The specificity of the human anti-cyt c autoantibodies appear to be similar to that of mouse anti-human cyt c antibodies and to antibodies elicited in mice against rat (mouse) cyt c.

Original languageEnglish (US)
Pages (from-to)1834-1840
Number of pages7
JournalJournal of Immunology
Volume144
Issue number5
StatePublished - 1990
Externally publishedYes

Fingerprint

Cytochromes c
Autoantibodies
Autoimmune Diseases
Antibodies
Connective Tissue Diseases
Autoantigens
Histones
Amino Acid Sequence
Animal Models
Pathology
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Mamula, M. J., Jemmerson, R., & Hardin, J. A. (1990). The specificity of human anti-cytochrome c autoantibodies that arise in autoimmune disease. Journal of Immunology, 144(5), 1834-1840.

The specificity of human anti-cytochrome c autoantibodies that arise in autoimmune disease. / Mamula, M. J.; Jemmerson, R.; Hardin, J. A.

In: Journal of Immunology, Vol. 144, No. 5, 1990, p. 1834-1840.

Research output: Contribution to journalArticle

Mamula, MJ, Jemmerson, R & Hardin, JA 1990, 'The specificity of human anti-cytochrome c autoantibodies that arise in autoimmune disease', Journal of Immunology, vol. 144, no. 5, pp. 1834-1840.
Mamula, M. J. ; Jemmerson, R. ; Hardin, J. A. / The specificity of human anti-cytochrome c autoantibodies that arise in autoimmune disease. In: Journal of Immunology. 1990 ; Vol. 144, No. 5. pp. 1834-1840.
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