The rotational order-disorder structure of the reversibly photoswitchable red fluorescent protein rsTagRFP

Sergei Pletnev, Fedor V. Subach, Vladislav V. Verkhusha, Zbigniew Dauter

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The rotational order-disorder (OD) structure of the reversibly photoswitchable fluorescent protein rsTagRFP is discussed in detail. The structure is composed of tetramers of 222 symmetry incorporated into the lattice in two different orientations rotated 90° with respect to each other around the crystal c axis and with tetramer axes coinciding with the crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates the rotational OD structure with statistically averaged I422 symmetry. Despite order-disorder pathology, the structure of rsTagRFP has electron-density maps of good quality for both non-overlapping and overlapping parts of the model. The crystal contacts, crystal internal architecture and a possible mechanism of rotational OD crystal formation are discussed.

Original languageEnglish (US)
Pages (from-to)31-39
Number of pages9
JournalActa Crystallographica Section D: Biological Crystallography
Volume70
Issue number1
DOIs
StatePublished - Jan 1 2014

Keywords

  • OD structure
  • fluorescent protein
  • rotational order-disorder

ASJC Scopus subject areas

  • Structural Biology

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