The role of the laminin β subunit in laminin heterotrimer assembly and basement membrane function and development in C. elegans

Gautam Kao, Cheng Chen Huang, Edward M. Hedgecock, David H. Hall, William G. Wadsworth

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Laminins are components of basement membranes that are required for morphogenesis, organizing cell adhesions and cell signaling. Studies have suggested that laminins function as αx βy γz heterotrimers in vivo. In C. elegans, there is only one laminin β gene, suggesting that it is required for all laminin functions. Our analysis is consistent with the role of the laminin β as a subunit of laminin heterotrimers; the same cells express the laminin α, β, and γ subunits, the laminin β subunit localizes to all basement membranes throughout development, and secretion of the β subunit requires an α subunit. RNAi inhibition of the β subunit gene or of the other subunit genes causes an embryonic lethality phenotype. Furthermore, a distinctive set of phenotypes is caused by both viable laminin α and β partial loss-of-function mutations. These results show developmental roles for the laminin β subunit, and they provide further genetic evidence for the importance of heterotrimer assembly in vivo.

Original languageEnglish (US)
Pages (from-to)211-219
Number of pages9
JournalDevelopmental Biology
Volume290
Issue number1
DOIs
StatePublished - Feb 1 2006

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Laminin
Basement Membrane
Genes
Phenotype
RNA Interference
Morphogenesis
Cell Adhesion
Mutation

Keywords

  • Basement membrane
  • C. elegans
  • Extracellular matrix
  • Laminin

ASJC Scopus subject areas

  • Developmental Biology

Cite this

The role of the laminin β subunit in laminin heterotrimer assembly and basement membrane function and development in C. elegans. / Kao, Gautam; Huang, Cheng Chen; Hedgecock, Edward M.; Hall, David H.; Wadsworth, William G.

In: Developmental Biology, Vol. 290, No. 1, 01.02.2006, p. 211-219.

Research output: Contribution to journalArticle

Kao, Gautam ; Huang, Cheng Chen ; Hedgecock, Edward M. ; Hall, David H. ; Wadsworth, William G. / The role of the laminin β subunit in laminin heterotrimer assembly and basement membrane function and development in C. elegans. In: Developmental Biology. 2006 ; Vol. 290, No. 1. pp. 211-219.
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