The role of macrophages in the production of lymphokines by T and B lymphocytes

S. M. Wahl, J. M. Wilton, David L. Rosenstreich, J. J. Oppenheim

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The role of macrophages in the production of 2 lymphokines, monocyte chemotactic factor and macrophage activating factor, was investigated. Lymphokine production by guinea pig lymph node and spleen cells required macrophages for thymus dependent antigens and mitogens. In contrast, B cell stimulants which also induce the synthesis of lymphokines were macrophage independent. When populations of relatively pure B or T lymphocytes were isolated, it was found that T cells required viable macrophage cooperation to product these 2 lymphokines and to undergo proliferation in response to specific antigens, whereas B cells could be directly activated in the absence of macrophages. These findings suggest that T and B cells have different requirements for activation and for macrophage cooperation. Furthermore, since lymphokine synthesis is evident within the first 4 hr of stimulant presentation, these observations demonstrate that macrophages play an essential role in the earliest events of lymphocyte activation.

Original languageEnglish (US)
Pages (from-to)1296-1301
Number of pages6
JournalJournal of Immunology
Volume114
Issue number4
StatePublished - 1975
Externally publishedYes

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Lymphokines
B-Lymphocytes
Macrophages
T-Lymphocytes
Macrophage-Activating Factors
Antigens
Macrophage Activation
Chemotactic Factors
Lymphocyte Activation
Mitogens
Thymus Gland
Monocytes
Guinea Pigs
Spleen
Lymph Nodes
Population

ASJC Scopus subject areas

  • Immunology

Cite this

The role of macrophages in the production of lymphokines by T and B lymphocytes. / Wahl, S. M.; Wilton, J. M.; Rosenstreich, David L.; Oppenheim, J. J.

In: Journal of Immunology, Vol. 114, No. 4, 1975, p. 1296-1301.

Research output: Contribution to journalArticle

Wahl, SM, Wilton, JM, Rosenstreich, DL & Oppenheim, JJ 1975, 'The role of macrophages in the production of lymphokines by T and B lymphocytes', Journal of Immunology, vol. 114, no. 4, pp. 1296-1301.
Wahl, S. M. ; Wilton, J. M. ; Rosenstreich, David L. ; Oppenheim, J. J. / The role of macrophages in the production of lymphokines by T and B lymphocytes. In: Journal of Immunology. 1975 ; Vol. 114, No. 4. pp. 1296-1301.
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