The regulatory effect of miRNAs is a heritable genetic trait in humans

Paul Geeleher, Stephanie R. Huang, Eric R. Gamazon, Aaron Golden, Cathal Seoighe

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: microRNAs (miRNAs) have been shown to regulate the expression of a large number of genes and play key roles in many biological processes. Several previous studies have quantified the inhibitory effect of a miRNA indirectly by considering the expression levels of genes that are predicted to be targeted by the miRNA and this approach has been shown to be robust to the choice of prediction algorithm. Given a gene expression dataset, Cheng et al. defined the regulatory effect score (RE-score) of a miRNA as the difference in the gene expression rank of targets of the miRNA compared to non-targeted genes.Results: Using microarray data from parent-offspring trios from the International HapMap project, we show that the RE-score of most miRNAs is correlated between parents and offspring and, thus, inter-individual variation in RE-score has a genetic component in humans. Indeed, the mean RE-score across miRNAs is correlated between parents and offspring, suggesting genetic differences in the overall efficiency of the miRNA biogenesis pathway between individuals. To explore the genetics of this quantitative trait further, we carried out a genome-wide association study of the mean RE-score separately in two HapMap populations (CEU and YRI). No genome-wide significant associations were discovered; however, a SNP rs17409624, in an intron of DROSHA, was significantly associated with mean RE-score in the CEU population following permutation-based control for multiple testing based on all SNPs mapped to the canonical miRNA biogenesis pathway; of 244 individual miRNA RE-scores assessed in the CEU, 214 were associated (p < 0.05) with rs17409624. The SNP was also nominally significantly associated (p = 0.04) with mean RE-score in the YRI population. Interestingly, the same SNP was associated with 17 (8.5% of all expressed) miRNA expression levels in the CEU. We also show here that the expression of the targets of most miRNAs is more highly correlated with global changes in miRNA regulatory effect than with the expression of the miRNA itself.Conclusions: We present evidence that miRNA regulatory effect is a heritable trait in humans and that a polymorphism of the DROSHA gene contributes to the observed inter-individual differences.

Original languageEnglish (US)
Article number383
JournalBMC Genomics
Volume13
Issue number1
DOIs
StatePublished - Aug 10 2012

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MicroRNAs
Single Nucleotide Polymorphism
HapMap Project
Gene Expression
Population
Genes
Biological Phenomena
Genome-Wide Association Study
Individuality
Introns

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

Geeleher, P., Huang, S. R., Gamazon, E. R., Golden, A., & Seoighe, C. (2012). The regulatory effect of miRNAs is a heritable genetic trait in humans. BMC Genomics, 13(1), [383]. https://doi.org/10.1186/1471-2164-13-383

The regulatory effect of miRNAs is a heritable genetic trait in humans. / Geeleher, Paul; Huang, Stephanie R.; Gamazon, Eric R.; Golden, Aaron; Seoighe, Cathal.

In: BMC Genomics, Vol. 13, No. 1, 383, 10.08.2012.

Research output: Contribution to journalArticle

Geeleher, P, Huang, SR, Gamazon, ER, Golden, A & Seoighe, C 2012, 'The regulatory effect of miRNAs is a heritable genetic trait in humans', BMC Genomics, vol. 13, no. 1, 383. https://doi.org/10.1186/1471-2164-13-383
Geeleher P, Huang SR, Gamazon ER, Golden A, Seoighe C. The regulatory effect of miRNAs is a heritable genetic trait in humans. BMC Genomics. 2012 Aug 10;13(1). 383. https://doi.org/10.1186/1471-2164-13-383
Geeleher, Paul ; Huang, Stephanie R. ; Gamazon, Eric R. ; Golden, Aaron ; Seoighe, Cathal. / The regulatory effect of miRNAs is a heritable genetic trait in humans. In: BMC Genomics. 2012 ; Vol. 13, No. 1.
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