The redox state of activated bleomycin

R. M. Burger, J. S. Blanchard, S. B. Horwitz, J. Peisach

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44 Scopus citations

Abstract

Activated bleomycin appears to have two more oxidizing equivalents than the Fe(III)·bleomycin to which it spontaneously decays. Activated bleomycin reacts with NADH and thio-NADH, two-electron reductants, and with KI, a one-electron reductant, to yield Fe(III)·bleomycin. The observed stoichiometries were 0.85 ± 0.07 eq of thio-NADH oxidized or 1.5 ± 0.25 eq of KI oxidized per mole of activated bleomycin. None of these reactions requires the presence of a redox mediator, as does the reduction of Fe(III)·bleomycin by NADH or thio-NADH. The oxidations of both pyridine nucleotide coenzymes and of KI are inhibited by DNA, the usual bleomycin target.

Original languageEnglish (US)
Pages (from-to)15406-15409
Number of pages4
JournalJournal of Biological Chemistry
Volume260
Issue number29
StatePublished - 1985

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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