The redox state of activated bleomycin

R. M. Burger, J. S. Blanchard, S. B. Horwitz, J. Peisach

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Activated bleomycin appears to have two more oxidizing equivalents than the Fe(III)·bleomycin to which it spontaneously decays. Activated bleomycin reacts with NADH and thio-NADH, two-electron reductants, and with KI, a one-electron reductant, to yield Fe(III)·bleomycin. The observed stoichiometries were 0.85 ± 0.07 eq of thio-NADH oxidized or 1.5 ± 0.25 eq of KI oxidized per mole of activated bleomycin. None of these reactions requires the presence of a redox mediator, as does the reduction of Fe(III)·bleomycin by NADH or thio-NADH. The oxidations of both pyridine nucleotide coenzymes and of KI are inhibited by DNA, the usual bleomycin target.

Original languageEnglish (US)
Pages (from-to)15406-15409
Number of pages4
JournalJournal of Biological Chemistry
Volume260
Issue number29
Publication statusPublished - Dec 1 1985

    Fingerprint

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this