Abstract
Activated bleomycin appears to have two more oxidizing equivalents than the Fe(III)·bleomycin to which it spontaneously decays. Activated bleomycin reacts with NADH and thio-NADH, two-electron reductants, and with KI, a one-electron reductant, to yield Fe(III)·bleomycin. The observed stoichiometries were 0.85 ± 0.07 eq of thio-NADH oxidized or 1.5 ± 0.25 eq of KI oxidized per mole of activated bleomycin. None of these reactions requires the presence of a redox mediator, as does the reduction of Fe(III)·bleomycin by NADH or thio-NADH. The oxidations of both pyridine nucleotide coenzymes and of KI are inhibited by DNA, the usual bleomycin target.
Original language | English (US) |
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Pages (from-to) | 15406-15409 |
Number of pages | 4 |
Journal | Journal of Biological Chemistry |
Volume | 260 |
Issue number | 29 |
State | Published - 1985 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology